Novel antiinflammatory biologics shaped by parasite-host coevolution.

Abstract

Parasitic helminth infections, while a major cause of neglected tropical disease burden, negatively correlate with the incidence of immune-mediated inflammatory diseases such as inflammatory bowel diseases (IBD). To evade expulsion, helminths have developed sophisticated mechanisms to regulate their host's immune responses. Controlled experimental human helminth infections have been assessed clinically for treating inflammatory conditions; however, such a radical therapeutic modality has challenges. An alternative approach is to harness the immunomodulatory properties within the worm's excretory-secretory (ES) complement, its secretome. Here, we report a biologics discovery and validation pipeline to generate and screen in vivo a recombinant cell-free secretome library of helminth-derived immunomodulatory proteins. We successfully expressed 78 recombinant ES proteins from gastrointestinal hookworms and screened the crude in vitro translation reactions for anti-IBD properties in a mouse model of acute colitis. After statistical filtering and ranking, 20 proteins conferred significant protection against various parameters of colitis. Lead candidates from distinct protein families, including annexins, transthyretins, nematode-specific retinol-binding proteins, and SCP/TAPS were identified. Representative proteins were produced in mammalian cells and further validated, including ex vivo suppression of inflammatory cytokine secretion by T cells from IBD patient colon biopsies. Proteins identified herein offer promise as novel, safe, and mechanistically differentiated biologics for treating the globally increasing burden of inflammatory diseases.

• Authors: Stephanie M Ryan, Roland Ruscher, Wayne A Johnston, Darren A Pickering, Malcolm W Kennedy, Brian O Smith, Linda Jones, Geraldine Buitrago, Matt A Field, Adrian J Esterman, Connor P McHugh, Daniel J Browne, Martha M Cooper, Rachael Y M Ryan, Denise L Doolan, Christian R Engwerda, Kim Miles, Makedonka Mitreva, John Croese, Tony Rahman, Kirill Alexandrov, Paul R Giacomin, Alex Loukas
• Journal: Proceedings of the National Academy of Sciences of the United States of America (Proc Natl Acad Sci U S A) Vol. 119 Issue 36 Pg. e2202795119 (09 06 2022) ISSN: 1091-6490 [Electronic] United States
• PMID: 36037362 (Publication Type: Journal Article)

Chemical References

• Anti-Inflammatory Agents
• Biological Products
• Helminth Proteins

Topics

• Animals
• Anti-Inflammatory Agents (pharmacology)
• Biological Products (pharmacology)
• Colitis (drug therapy)
• Helminth Proteins (genetics, pharmacology)
• Helminths
• Humans
• Inflammatory Bowel Diseases (drug therapy, parasitology)
• Mice