Abstract | ETHNOPHARMACOLOGICAL RELEVANCE: AIM OF THE STUDY: To determine the protective roles and mechanism of FTZ in mouse renal injury and fibrosis under hyperuricemic condition. MATERIALS AND METHODS: HN mice, induced by potassium oxonate and hypoxanthine, were administrated with 600 and 1200 mg/kg FTZ (intragastrically) daily for three weeks. SUA levels, renal functions and histological changes were analyzed. Western blotting, quantitative real-time PCR (q-PCR) and RNA sequencing were used to identify the roles and underlying mechanism of FTZ in HN mice. RESULTS: We demonstrated that FTZ treatment mitigated renal injury in mice, as evidenced by the decrease in SUA, serum creatinine (SCr) and cystatin C (Cys C) levels, as well as improved renal histology. FTZ markedly attenuates inflammasome activation, collagen deposition and the imbalance of uric acid transporters. RNA-sequencing revealed a key mechanism involved in the protective effects on HN mice was related to PI3K/AKT/NF-κB pathway. Western blot also confirmed that FTZ diminished the phosphorylation of AKT and p65 in HN mice. CONCLUSIONS:
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Authors | Ming-Hui Li, Jin Guan, Zhe Chen, Ju-Xian Mo, Kai-Reng Wu, Xu-Guang Hu, Tian Lan, Jiao Guo |
Journal | Journal of ethnopharmacology
(J Ethnopharmacol)
Vol. 298
Pg. 115644
(Nov 15 2022)
ISSN: 1872-7573 [Electronic] Ireland |
PMID | 35987412
(Publication Type: Journal Article)
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Copyright | Copyright © 2022 Elsevier B.V. All rights reserved. |
Chemical References |
- NF-kappa B
- Uric Acid
- Proto-Oncogene Proteins c-akt
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Topics |
- Animals
- Fibrosis
- Hyperuricemia
- Inflammation
(drug therapy)
- Kidney
- Mice
- NF-kappa B
(metabolism)
- Phosphatidylinositol 3-Kinases
(metabolism)
- Proto-Oncogene Proteins c-akt
(metabolism)
- Uric Acid
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