A Two-Photon Fluorescent Probe for the Visual Detection of Peroxynitrite in Living Cells and Zebrafish.

Abstract

Peroxynitrite (ONOO-), as an important reactive oxygen species (ROS), holds great potential to react with a variety of biologically active substances, leading to the occurrence of various diseases such as cancer and neurodegenerative diseases. In this work, we developed a novel mitochondria-localized fluorescent probe, HDBT-ONOO-, which was designed as a mitochondria-targeting two-photon fluorescence probe based on 1,8-naphthylimide fluorophore and the reactive group of 4-(bromomethyl)-benzene boronic acid pinacol ester. More importantly, the probe exhibited good biocompatibility, sensitivity, and selectivity, enabling its successful application in imaging the generation of intracellular and extracellular ONOO-. Furthermore, exogenous and endogenous ONOO- products in live zebrafish were visualized. It is greatly expected that the designed probe can serve as a useful imaging tool for clarifying the distribution and pathophysiological functions of ONOO- in cells and zebrafish.

• Authors: Zhencai Xu, Jin Qian, Yufeng Ge, Yalan Wang, Hongwei Chen
• Journal: Molecules (Basel, Switzerland) (Molecules) Vol. 27 Issue 15 (Jul 29 2022) ISSN: 1420-3049 [Electronic] Switzerland
• PMID: 35956806 (Publication Type: Journal Article)

Chemical References

• Boronic Acids
• Fluorescent Dyes
• Peroxynitrous Acid

Topics

• Animals
• Boronic Acids
• Fluorescent Dyes
• Peroxynitrous Acid
• Photons
• Zebrafish