Abstract | OBJECTIVE: Periodontal ligament cells (PDLCs) are critical for wound healing and regenerative capacity of periodontal diseases. Within an inflammatory periodontal pocket, a hypoxic environment can aggravate periodontal inflammation, where PDLCs response to the inflammation would change. Resolvin D1 (RvD1) is an endogenous lipid mediator, which can impact intracellular inflammatory pathways of periodontal/oral cells and periodontal regeneration. It is not clear how hypoxia and RvD1 impact the inflammatory responses of pro-inflammatory PDLCs phenotype. Therefore, this study aimed to test hypoxia could induce changes in pro-inflammatory phenotype of PDLCs and RvD1 could reverse it. METHODS: Human PDLCs were cultured from periodontal tissues from eight healthy individuals and were characterized by immunofluorescence staining of vimentin and cytokeratin. Cell viability was examined by Methyl-thiazolyl-tetrazolium (MTT) assay. To examine the effects of hypoxia and RvD1 on the inflammatory responses of pro-inflammatory PDLCs phenotype, protein levels and gene expressions of inflammatory cytokines and signal transduction molecules were measured by enzyme-linked immunosorbent assay (ELISA), western blotting (WB), and real-time quantitative reverse transcription PCR (real-time qRT-PCR). Alizarin red S staining and real-time qRT-PCR were employed to study the effects of hypoxia and RvD1 on the osteogenic differentiation of pro-inflammatory PDLCs phenotype. RESULTS: CONCLUSION: Our results indicate that hypoxia up-regulated the inflammatory level of PDLCs. RvD1 can reduce under- hypoxia-induced pro-inflammatory cytokines in the inflammatory phenotype of PDLCs. Moreover, RvD1 promotes the calcium nodules in PDLCs, possibly by affecting the p38 MAPK signaling pathway through Akt and HIF-1α.
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Authors | Jiazheng Cai, Jing Liu, Jing Yan, Xuexia Lu, Xiaoli Wang, Si Li, Kamal Mustafa, Huihui Wang, Ying Xue, Manal Mustafa, Alpdogan Kantarci, Zhe Xing |
Journal | Journal of periodontal research
(J Periodontal Res)
Vol. 57
Issue 5
Pg. 1034-1042
(Oct 2022)
ISSN: 1600-0765 [Electronic] United States |
PMID | 35944267
(Publication Type: Journal Article)
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Copyright | © 2022 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. |
Chemical References |
- resolvin D1
- Docosahexaenoic Acids
- Proto-Oncogene Proteins c-akt
- p38 Mitogen-Activated Protein Kinases
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Topics |
- Cells, Cultured
- Docosahexaenoic Acids
- Humans
- Hypoxia
- Inflammation
- Osteogenesis
- Periodontal Ligament
- Phenotype
- Proto-Oncogene Proteins c-akt
- p38 Mitogen-Activated Protein Kinases
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