First Characterization of Chicken Interleukin-9.

Abstract

Interleukin-9 (IL-9) is a pleiotropic cytokine that acts on a variety of cells and tissues, and plays roles in inflammation and infection as well as tumor immunity. While mammalian IL-9s have been widely investigated, avian IL-9 has not yet been identified and characterized. In this study, we cloned chicken IL-9 (chIL-9) and performed a phylogenetic analysis, examined its tissue distribution, characterized the biological functions of recombinant chIL-9 (rchIL-9) and the expression form of natural chIL-9. Phylogenetic analysis showed that chIL-9 has less than 30% amino acid identity with mammalian IL-9s. The chIL-9 mRNA can be abundantly detected only in the testis and thymus, and are significantly up-regulated in peripheral blood mononuclear cells (PBMCs) upon mitogen stimulation. The rchIL-9 was produced by prokaryotic and eukaryotic expression systems and showed biological activity in activating monocytes/macrophages to produce inflammatory cytokines and promoting the proliferation of CD3+ T cells. In addition, four monoclonal antibodies (mAbs) and rabbit polyclonal antibody (pAb) against rchIL-9 were generated. Using anti-chIL-9 mAbs and pAb, natural chIL-9 expressed by the activated PBMCs of chickens with a molecular weight of 25kD was identified by Western-blotting. Collectively, our study reveals for the first time the presence of functional IL-9 in birds and lays the ground for further investigating the roles of chIL-9 in diseases and immunity.

• Authors: Shuangjiang He, Lina Chen, Xiaoli Hao, Yi Yang, Shaobin Shang
• Journal: Frontiers in immunology (Front Immunol) Vol. 13 Pg. 889991 ( 2022) ISSN: 1664-3224 [Electronic] Switzerland
• PMID: 35795670 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2022 He, Chen, Hao, Yang and Shang.

Chemical References

• Antibodies, Monoclonal
• Cytokines
• Interleukin-9

Topics

• Animals
• Antibodies, Monoclonal
• Chickens
• Cytokines (genetics)
• Interleukin-9 (genetics)
• Leukocytes, Mononuclear
• Mammals
• Phylogeny
• Rabbits