Cytokine response to Ancylostoma duodenale (A. duodenale)
infection was measured after starting treatments with
piperazine. This study aims to determine the impact of
cytokine production after
infection with A. duodenale before and
after treatment with
piperazine. Blood and stool samples of 50 patients with A. duodenale
infection and 28 healthy individuals (control) were collected. In this study, IFNγ,
IL-5,
IL-12, and
IL-13 in serum (using ELISA-based methods) were measured. Stool samples were examined using the Kato-Katz technique to detect A. duodenale parasites. Blood and stool samples were analyzed 14 days after starting
piperazine treatment for A. duodenale
infection. The medium concentration of IFNγ,
IL-5,
IL-12, and
IL-13 in the serum samples with A. duodenale
infection is higher than that of the control group. IFNγ,
IL-5,
IL-12, and
IL-13 levels were significantly higher in the infected individuals (10.5±7.4 pg/ml, 14.6±5.1 pg/ml, 8.5±3.2 pg/ml and 13.6±7.5 pg/ml respectively) than the control group (4.7±2.4 pg/
ml, 7.8±4.06 pg/ml, 6.3±3.4 pg/ml and 3.5±2.7 pg/ml respectively). Also,
piperazine treatment can significantly reduce
cytokines levels (IFN-γ: P=0.043, IL-5: P=0.02, and
IL-12, p=0.001). This study shows that
piperazine treatment can reduce
cytokines profiles in patients with A. duodenale
infection.