Abstract | PURPOSE: MATERIALS AND METHODS: AgNPs were synthesized and characterized by transmission electron microscopy, UV-vis spectrometry, thermogravimetric analyses, and dynamic light scattering. We determined the safety and the antimicrobial and anti-inflammatory properties of synthesized AgNPs in an in vitro model of periodontitis. Antimicrobial properties were determined by measuring the minimum inhibitory concentration (MIC) and minimum biofilm eradication concentration (MBEC) on reference strains of bacteria and fungi. Human gingival fibroblast (HGF-1), murine macrophage (RAW264.7) and human foetal osteoblast (hFOB1.19) cells were used in the study. Lipopolysaccharide (LPS) was used to induce inflammation. Cytokine levels were measured using an enzyme-linked immunosorbent assay; metalloproteinase expression was measured using Western blotting. RESULTS: The synthesized AgNPs were spherical and narrow-dispersed with an average diameter of 13.4 nm ± 3.0 nm in the case of AgNPs-CHL and 3.72 nm ± 0.72 nm in the case of AgNPs-PEG-MET. Both types of AgNPs were active against bacteria and fungi. AgNPs-CHL proved to be a more potent antimicrobial agent, although they were more cytotoxic than AgNPs-PEG-MET; however, both demonstrated beneficial properties in nontoxic concentrations. AgNPs-CHL and AgNPs-PEG-MET decreased the production of proinflammatory cytokines IL-1β, IL-6, IL-8 and TNFα. Both agents also decreased the levels of metalloproteinases MMP3 and MMP8, which may indicate that they will inhibit tissue degradation. CONCLUSION: AgNPs-CHL and AgNPs-PEG-MET may be possible therapeutic options for PD, as they have antibacterial and anti-inflammatory properties. However, to fully understand the potential of AgNPs, our in vitro findings must be evaluated in an in vivo model.
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Authors | Karol P Steckiewicz, Piotr Cieciórski, Ewelina Barcińska, Maciej Jaśkiewicz, Magdalena Narajczyk, Marta Bauer, Wojciech Kamysz, Elżbieta Megiel, Iwona Inkielewicz-Stepniak |
Journal | International journal of nanomedicine
(Int J Nanomedicine)
Vol. 17
Pg. 495-517
( 2022)
ISSN: 1178-2013 [Electronic] New Zealand |
PMID | 35140461
(Publication Type: Journal Article)
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Copyright | © 2022 Steckiewicz et al. |
Chemical References |
- Pharmaceutical Preparations
- Metronidazole
- Silver
- Chlorhexidine
|
Topics |
- Animals
- Chlorhexidine
- Humans
- Metal Nanoparticles
- Metronidazole
- Mice
- Periodontitis
(drug therapy)
- Pharmaceutical Preparations
- Silver
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