Detection of individual
cytokines in routine biopsies from patients with inflammatory
skin diseases has the potential to personalize diagnosis and treatment selection, but this approach has been limited by technical feasibility. We evaluate whether a chromogen-based
RNA in situ hybridization approach can be used to detect druggable
cytokines in
psoriasis and
atopic dermatitis. A series of
psoriasis (n = 20) and
atopic dermatitis (n = 26) biopsies were stained using
RNA in situ hybridization for
IL4, IL12B (
IL-12/23 p40),
IL13, IL17A, IL17F, IL22, IL23A (IL-23 p19), IL31, and TNF (TNF-α). NOS2 and IFNG, canonical
psoriasis biomarkers, were also included. All 20 of the
psoriasis cases were positive for IL17A, which tended to be the predominant
cytokine, although some cases had relatively higher levels of IL12B, IL17F, or IL23A. The majority of
cytokine expression in
psoriasis was epidermal. A total of 22 of 26
atopic dermatitis cases were positive for
IL13, also at varying levels; a subset of cases had significant
IL4, IL22, or IL31 expression. Patterns were validated in independent bulk
RNA-sequencing and single-cell
RNA-sequencing datasets. Overall,
RNA in situ hybridization for
cytokines appears highly specific with virtually no background staining and may allow for individualized evaluation of treatment-relevant
cytokine targets in biopsies from patients with inflammatory skin disorders.