Evodiamine, an
alkaloid component in the fruit of Evodia, has been shown to have biological functions such as
antioxidant and anti-inflammatory. But whether
evodiamine plays an improvement role on
mastitis has not been studied. To investigate the effect and mechanism of
evodiamine on
lipopolysaccharide (LPS)-induced
mastitis was the purpose of this study. In animal experiments, the mouse
mastitis model was established by injecting LPS into the canals of the mammary gland. The results showed that
evodiamine could significantly relieve the pathological injury of breast tissue and the production of pro-inflammatory
cytokines and inhibit the activation of
inflammation-related pathways such as AKT, NF-κB p65, ERK1/2, p38, and JNK. In cell experiments, the mouse mammary epithelial cells (mMECs) were incubated with
evodiamine for 1 h and then stimulated with LPS. Next, pro-inflammatory mediators and
inflammation-related signal pathways were detected. As expected, our results showed that
evodiamine notably ameliorated the inflammatory reaction and inhibit the activation of related signaling pathways of mMECs. All the results suggested that
evodiamine inhibited
inflammation by inhibiting the phosphorylation of AKT, NF-κBp65, ERK1/2, p38, and JNK thus the LPS-induced
mastitis was ameliorated. These findings suggest that
evodiamine maybe a potential drug for
mastitis because of its anti-inflammatory effects.