Abstract | INTRODUCTION: OBJECTIVES: We profiled the metabolomic changes in plasma of ALL patients initial- and post- induction therapy. METHODS: We exploited a biorepository of non-fasted plasma samples derived from the Dana Farber Cancer Institute ALL Consortium; these samples were obtained from 50 ALL patients initial- and post-induction therapy. Plasma metabolites and complex lipids were analyzed by high resolution tandem mass spectrometry and differential mobility tandem mass spectrometry. Data were analyzed using a covariate-adjusted regression model with multiplicity adjustment. Pathway enrichment analysis and co-expression network analysis were performed to identify unique clusters of molecules. RESULTS: More than 1200 metabolites and complex lipids were identified in the total of global metabolomics and lipidomics platforms. Over 20% of those molecules were significantly altered. In the pathway enrichment analysis, lipids, particularly phosphatidylethanolamines (PEs), were identified. Network analysis indicated that the bioactive fatty acids, docosahexaenoic acid (DHA)-containing (22:6) triacylglycerols (TAGs), were decreased in the post-induction therapy. CONCLUSION: Metabolomic profiling in ALL patients revealed a large number of alterations following induction chemotherapy. In particular, lipid metabolism was substantially altered. The changes in metabolites and complex lipids following induction therapy could provide insight into the adverse events experienced by ALL patients.
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Authors | Toshie Saito, Yue Wei, Li Wen, Chaitanya Srinivasan, Benjamin O Wolthers, Cheng-Yu Tsai, Marian H Harris, Kristen Stevenson, Craig Byersdorfer, Judy-April Oparaji, Christian Fernandez, Amitava Mukherjee, Maisam Abu-El-Haija, Sameer Agnihotri, Kjeld Schmiegelow, Megan R Showalter, Paul W Fogle, Scott McCulloch, Kevin Contrepois, Lewis B Silverman, Ying Ding, Sohail Z Husain |
Journal | Metabolomics : Official journal of the Metabolomic Society
(Metabolomics)
Vol. 17
Issue 7
Pg. 64
(06 27 2021)
ISSN: 1573-3890 [Electronic] United States |
PMID | 34175981
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Chemical References |
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Topics |
- Child
- Humans
- Induction Chemotherapy
- Lipids
- Metabolomics
- Precursor Cell Lymphoblastic Leukemia-Lymphoma
(drug therapy)
- Tandem Mass Spectrometry
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