Abstract | BACKGROUND: The pathogenesis of lupus nephritis (LN) remains not fully understood. In this study, we aimed to explore the pathogenic roles of autoantibodies against human renal glomerular endothelial cells (HRGEC) in LN patients. METHODS: RESULTS: LN patients had higher serum levels of IgG anti-HRGEC antibodies than SLE patients without LN and healthy controls. Four monoclonal IgG anti-HRGEC antibodies (LN1-4) were obtained; LN1 and LN2 were IgG3 while LN3 and LN4 were IgG1. Among these monoclonal antibodies, LN1-3 were cross-reactive with dsDNA. The functional assays showed that compared with IgG1/ IgG3 isotype controls, LN3 had an effect on HRGEC to enhance interleukin (IL)-6 production, LN4 could enhance IL-8 and monocyte chemoattractant protein (MCP)-1 production, and LN1-3 possessed the ability to induce interferon (IFN)-α production by HRGEC. Moreover, the removal of DNA on the HRGEC surface by DNAse 1 did not interpose the binding of LN1-3 to HRGEC and the effects of LN1-3 on IFN-α induction by HRGEC. CONCLUSIONS: Some IgG anti-HRGEC antibodies in LN patients had the ability to enhance endothelial proinflammatory cytokine (IL-6, IL-8, and MCP-1) production, and some could induce the DNA-independent production of IFN-α by HRGEC.
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Authors | Ya-Chiao Hu, I-Jung Tsai, Hui-Yao Hsu, Bor-Luen Chiang, Yao-Hsu Yang |
Journal | Arthritis research & therapy
(Arthritis Res Ther)
Vol. 23
Issue 1
Pg. 171
(06 16 2021)
ISSN: 1478-6362 [Electronic] England |
PMID | 34134755
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibodies, Monoclonal
- Autoantibodies
- Interferon-alpha
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Topics |
- Antibodies, Monoclonal
- Autoantibodies
- Endothelial Cells
- Humans
- Interferon-alpha
- Lupus Erythematosus, Systemic
- Lupus Nephritis
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