Maresin 1 alleviates the inflammatory response, reduces oxidative stress and protects against cardiac injury in LPS-induced mice.

Abstract

BACKGROUND:

Maresin 1 (MaR1) is a pro-resolving lipid mediator that has been reported to have strong regulatory effects on oxidative stress and inflammation. This study aimed to determine the effect of MaR1 on lipopolysaccharide (LPS)-induced sepsis-related cardiac injury and explore its possible mechanisms.

METHODS:

Mice were administered MaR1 or PBS and then treated with LPS or saline for 6 h. Then, cardiac function, cardiac injury markers, cardiac macrophage differentiation, oxidative stress and myocardial cell apoptosis in each group were measured.

RESULTS:

MaR1 treatment significantly decreased the serum levels of lactate dehydrogenase (LDH) and kinase isoenzyme (CK-MB) and improved cardiac function in LPS-induced mice. Treatment with MaR1 also inhibited LPS-induced M1 macrophage differentiation and reduced M1 macrophage-related cytokine secretion while promoting M2 macrophage differentiation and increasing M2 macrophage-related inflammatory mediator expression. In addition, MaR1 decreased serum malondialdehyde (MDA) levels and increased serum levels of superoxide dismutase (SOD) and glutathione (GSH), as well as cardiac expression of nuclear factor erythroid-2 related factor 2 (Nrf-2) and heme oxygenase 1 (HO-1), in LPS-induced mice. Furthermore, fewer TUNEL-positive cells were observed in the LPS + MaR1 group than in the LPS group.

CONCLUSIONS:

Our experimental results show that MaR1 alleviates cardiac injury and protects against cardiac dysfunction and may be beneficial in reducing sepsis-induced cardiac injury.

• Authors: Dan Li, Menglong Wang, Jing Ye, Jishou Zhang, Yao Xu, Zhen Wang, Mengmeng Zhao, Di Ye, Jun Wan
• Journal: Life sciences (Life Sci) Vol. 277 Pg. 119467 (Jul 15 2021) ISSN: 1879-0631 [Electronic] Netherlands
• PMID: 33811894 (Publication Type: Journal Article)
• Copyright: Copyright © 2021 Elsevier Inc. All rights reserved.

Chemical References

• 7,14-dihydroxydocosa-4,8,10,12,16,19-hexaenoic acid
• Lipopolysaccharides
• Protective Agents
• Docosahexaenoic Acids
• L-Lactate Dehydrogenase

Topics

• Animals
• Apoptosis (drug effects)
• Cell Differentiation (drug effects)
• China
• Docosahexaenoic Acids (metabolism, pharmacology)
• Heart Injuries (drug therapy, metabolism)
• Inflammation (metabolism)
• L-Lactate Dehydrogenase (metabolism)
• Lipopolysaccharides (adverse effects, pharmacology)
• Macrophages (metabolism)
• Mice
• Mice, Inbred C57BL
• Oxidative Stress (drug effects)
• Protective Agents (pharmacology)
• Sepsis (drug therapy, metabolism)