A systematic strategy for uncovering quality marker of Asari Radix et Rhizoma on alleviating inflammation based chemometrics analysis of components.

Abstract

Asari Radix et Rhizoma (Asarum), a traditional Chinese medicine (TCM), has been applied in clinical generally. However, due to the lack of valid methods for Asarum quality control, inhomogenous quality and therapy issues have become severe with each passing day. In this study, we aimed to establish a comprehensive multi-system to explore the quality control markers underlying pharmaceutical effects based on chemometrics analysis on the total ingredients of Asarum. In brief, DNA barcoding technology was used to screen out the unadulterated herbs in the 15 batches Asarum collected from different origins. Then, the chemical profiles of volatile/nonvolatile components of 10 batches Asarum with definite resource were obtained by HPLC Q-TOF/MS and GC/MS. Combination with chemometrics methods, 14 characteristic ingredients and 4 qualitative and quantitative markers were figured out preliminarily. Moreover, correlation analysis between the characteristic ingredients and the cytokines integrating the virtual targets prediction of network pharmacology, 3 potential bioactive substance were ascertained. In conclusion, l-asarinin, 2-Methoxy-4-vinylphenol and safrole were considered as the potent candidates for quality control markers based on the comprehensive understanding for therapeutic effects and the chemical information of Asarum, which provided a novel perspective of the development for the quality control of TCM.

• Authors: Yiwen Zhang, Saiyu Li, Yuting Liang, Ran Liu, Xinyan Lv, Qian Zhang, Huarong Xu, Kaishun Bi, Zuojing Li, Qing Li
• Journal: Journal of chromatography. A (J Chromatogr A) Vol. 1642 Pg. 461960 (Apr 12 2021) ISSN: 1873-3778 [Electronic] Netherlands
• PMID: 33684872 (Publication Type: Journal Article)
• Copyright: Copyright © 2021 Elsevier B.V. All rights reserved.

Chemical References

• Anti-Inflammatory Agents, Non-Steroidal
• Cytokines
• Drugs, Chinese Herbal
• Oils, Volatile
• asari radix et rhizoma

Topics

• Animals
• Anti-Inflammatory Agents, Non-Steroidal (analysis, pharmacology, therapeutic use)
• Asarum (chemistry)
• Chromatography, High Pressure Liquid
• Cytokines (analysis)
• DNA Barcoding, Taxonomic
• Discriminant Analysis
• Drugs, Chinese Herbal (analysis, pharmacology, therapeutic use)
• Gas Chromatography-Mass Spectrometry
• Inflammation (drug therapy)
• Least-Squares Analysis
• Male
• Mice
• Oils, Volatile (analysis)
• Phylogeny