The safety and efficacy of BCG encapsulated alginate particle (BEAP) against M.tb H37Rv infection in Macaca mulatta : A pilot study.

Abstract

Due to the limited utility of Bacillus Calmette-Guérin (BCG), the only approved vaccine available for tuberculosis, there is a need to develop a more effective and safe vaccine. We evaluated the safety and efficacy of a dry powder aerosol (DPA) formulation of BCG encapsulated alginate particle (BEAP) and the conventional intradermal BCG immunization in infant rhesus macaques (Macaca mulatta). The infant macaques were immunized intratracheally with DPA of BEAP into the lungs. Animals were monitored for their growth, behaviour, any adverse and allergic response. The protective efficacy of BEAP was estimated by the ex-vivo H37Rv infection method. Post-immunization with BEAP, granulocytes count, weight gain, chest radiography, levels of liver secreted enzymes, cytokines associated with inflammation like TNF and IL-6 established that BEAP is non-toxic and it does not elicit an allergic response. The T cells isolated from BEAP immunized animals' blood, upon stimulation with M.tb antigen, secreted high levels of IFN-γ, TNF, IL-6 and IL-2. The activated T cells from BEAP group, when co-cultured with M.tb infected macrophages, eliminated largest number of infected macrophages compared to the BCG and control group. This study suggests the safety and efficacy of BEAP in Non-human primate model.

• Authors: Ashwani Kesarwani, Parul Sahu, Kshama Jain, Prakriti Sinha, K Varsha Mohan, Puja S Nagpal, Surender Singh, Rana Zaidi, Perumal Nagarajan, Pramod Upadhyay
• Journal: Scientific reports (Sci Rep) Vol. 11 Issue 1 Pg. 3049 (02 04 2021) ISSN: 2045-2322 [Electronic] England
• PMID: 33542363 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Alginates
• BCG Vaccine
• Interleukin-2
• Interleukin-6
• Tuberculosis Vaccines
• Tumor Necrosis Factor-alpha
• Interferon-gamma

Topics

• Alginates (chemistry, pharmacology)
• Animals
• BCG Vaccine (adverse effects, pharmacology)
• Disease Models, Animal
• Humans
• Immunization
• Interferon-gamma (genetics)
• Interleukin-2 (genetics)
• Interleukin-6 (genetics)
• Macaca mulatta (immunology, microbiology)
• Mycobacterium tuberculosis (immunology, pathogenicity)
• T-Lymphocytes (drug effects, immunology)
• Tuberculosis (genetics, immunology, microbiology, prevention & control)
• Tuberculosis Vaccines (immunology, pharmacology)
• Tumor Necrosis Factor-alpha (genetics)