Astragaloside IV alleviates PM2.5-induced lung injury in rats by modulating TLR4/MyD88/NF-κB signalling pathway.

Abstract

OBJECTIVE:

Astragaloside IV (AS IV) is antioxidant and anti-inflammatory product, which is extracted from the Chinese herb Astragalus membranaceus. It is widely used in a variety of inflammatory diseases. The research was to explored the protective effects of AS IV against lung injury induced by particulate matter 2.5 (PM2.5) in vivo.

SUBJECTS AND METHODS:

Thirty-five male Sprague-Dawley rats were randomly divided into five groups (n=7 per group). (1) Normal saline group (NS), (2) AS IV group (AS) (100 mg/kg), (3) PM2.5 group (PM2.5), (4) PM2.5 + AS IV group (ASL) (50 mg/kg), and (5) PM2.5 + AS IVgroup (ASH) (100 mg/kg). Rats were pre-treated with AS IV intraperitoneally (50 and 100 mg/kg/day) for three days. Then, PM2.5 (7.5 mg/kg) was given by intratracheal instillation to induce lung injury. Six hours after PM2.5 stimulation, the rats were euthanized. Bronchoalveolar lavage fluid (BALF) was collected for assay of cytokines. Lung tissue was collected for oxidative stress, histology, immunohistochemistry, transmission electron microscope, and western blot analyses.

RESULTS:

AS IV alleviated PM2.5-induced lung injury by decreasing lung dry-wet ratio, reducing the level of interleukin-6 (IL-6), tumour necrosis factor-alpha (TNF-α), and C-reactive protein (CRP) in BALF, and reduced oxidative stress response in lung tissue. Western blot results revealed that AS-IV regulated the expression of TLR4/MyD88/NF-κB pathway proteins in lung tissues.

CONCLUSION:

AS IV mitigated PM2.5 induced lung injury by regulating the activity of TLR4/MyD88/NF-κB signalling pathway, reducing inflammatory and oxidative stress responses.

• Authors: Yongcan Wu, Wei Xiao, Caixia Pei, Mingjie Wang, Xiaomin Wang, Demei Huang, Fei Wang, Zhenxing Wang
• Journal: International immunopharmacology (Int Immunopharmacol) Vol. 91 Pg. 107290 (Feb 2021) ISSN: 1878-1705 [Electronic] Netherlands
• PMID: 33383446 (Publication Type: Journal Article)
• Copyright: Copyright © 2020 Elsevier B.V. All rights reserved.

Chemical References

• Anti-Inflammatory Agents
• Antioxidants
• Cytokines
• Inflammation Mediators
• Myd88 protein, rat
• Myeloid Differentiation Factor 88
• NF-kappa B
• Particulate Matter
• Saponins
• Tlr4 protein, rat
• Toll-Like Receptor 4
• Triterpenes
• astragaloside A

Topics

• Animals
• Anti-Inflammatory Agents (pharmacology)
• Antioxidants (pharmacology)
• Cytokines (metabolism)
• Disease Models, Animal
• Inflammation Mediators (metabolism)
• Lung (drug effects, metabolism, ultrastructure)
• Lung Injury (chemically induced, metabolism, pathology, prevention & control)
• Male
• Myeloid Differentiation Factor 88 (metabolism)
• NF-kappa B (metabolism)
• Oxidative Stress (drug effects)
• Particulate Matter
• Pulmonary Edema (chemically induced, metabolism, pathology, prevention & control)
• Rats, Sprague-Dawley
• Saponins (pharmacology)
• Signal Transduction
• Toll-Like Receptor 4 (metabolism)
• Triterpenes (pharmacology)