Abstract | Purpose: Methods: C57BL/6 mice subjected to desiccating stress (DS) were treated with 0.05% MZR in phosphate-buffered saline (PBS) or Restasis eye drops four times a day for 5 days. Untreated mice served as control. Tear secretion, Oregon green dextran staining, and the conjunctival goblet cell quantity were evaluated. The apoptosis and matrix metalloproteinase 9 (MMP-9) in the ocular surface, conjunctival CD4, and T helper-related cytokines were verified. The ocular tolerance of these two drugs was evaluated by observing the mice's behavioral changes. Results:
Topical administrations of MZR or Restasis both increased tear production, maintained goblet cell density, and improved corneal barrier function. Both MZR and Restasis suppressed the expression of MMP-9 and apoptosis in the ocular surface. Meanwhile, both MZR and Restasis decreased the infiltration of CD4+ T cells, reversed the production of interferon-γ, interleukin (IL)-17A, and IL-13 in conjunctiva under DS. The abovementioned efficacies between these two eye drops were not statistically significant. However, the number of scratching and wiping behaviors in the MZR-treated group was significantly less than in the Restasis-treated group. Conclusions: MZR (0.05% in PBS) could be a good competitive product for Restasis because of the comparable treatment effect in dry eye diseases and better ocular tolerability in ocular itch and pain. Translational Relevance:
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Authors | Xiang Lin, Yang Wu, Liying Tang, Weijie Ouyang, Yiran Yang, Zhaolin Liu, Jieli Wu, Xiaoxiang Zheng, Caihong Huang, Yueping Zhou, Xiaobo Zhang, Yongxiong Chen, Wei Li, Jiaoyue Hu, Zuguo Liu |
Journal | Translational vision science & technology
(Transl Vis Sci Technol)
Vol. 9
Issue 13
Pg. 22
(12 2020)
ISSN: 2164-2591 [Electronic] United States |
PMID | 33364077
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright 2020 The Authors. |
Chemical References |
- Ribonucleosides
- mizoribine
- Cyclosporine
|
Topics |
- Animals
- Cyclosporine
- Dry Eye Syndromes
(chemically induced)
- Mice
- Mice, Inbred C57BL
- Oregon
- Ribonucleosides
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