D-Carvone inhibit cerebral ischemia/reperfusion induced inflammatory response TLR4/NLRP3 signaling pathway.

Abstract

To explore the present treatment strategies for ischemic stroke lowered by ischemia-reperfusion (I/R) injury, to hypothesize the effect of d-Carvone on cerebral I/R brain injury induced neuroinflammation through oxidative stress markers mechanism via NRLP3 and TLR4 marker expressions in rat model. The rats were divided into four groups: Sham, I/R vehicle, I/R + D-carvone (10 mg/kg/bw), I/R + D-carvone (20 mg/kg/bw). Supplementation of d-carvone at dose of 10 and 20 m/kg/bw increased the water content, reduced infract volume, attenuated neurological score depicts, furthermore it had antioxidative, anti-inflammatory, and anti-apoptotic effects against cerebral I/R brain injury. In the brain tissues decreased proinflammatory cytokines IL-1β and TNF-α reduced interleukins IL-6, IL-4, IL-10 & VEGF dose dependently, and mRNA expressions of NLRP3, caspase -1, TNF-α, ASC, IL-1β and TLR3 down regulated in cerebral I/R induced rats. Finally d- carvone can successfully improve the cerebral I/R induced rats neuroinflammation, in the hippocampus and cortical areas of the brain finally reduces cerebral I/R induced injury. These results were hypothesized that d-carvone contributed to cerebral stroke associated with the TLR3, giving an excellent therapeutic approach for cerebral I/R brain injury.

• Authors: Mengyuan Dai, Lixiu Wu, Kunqiang Yu, Ri Xu, Yanbin Wei, Arunachalam Chinnathambi, Tahani Awad Alahmadi, Minya Zhou
• Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie (Biomed Pharmacother) Vol. 132 Pg. 110870 (Dec 2020) ISSN: 1950-6007 [Electronic] France
• PMID: 33080468 (Publication Type: Journal Article)
• Copyright: Copyright © 2020 The Authors. Published by Elsevier Masson SAS.. All rights reserved.

Chemical References

• Anti-Inflammatory Agents
• Antioxidants
• Cyclohexane Monoterpenes
• Cytokines
• Inflammation Mediators
• NLR Family, Pyrin Domain-Containing 3 Protein
• Neuroprotective Agents
• Nlrp3 protein, rat
• Tlr4 protein, rat
• Toll-Like Receptor 4
• carvone

Topics

• Animals
• Anti-Inflammatory Agents (pharmacology)
• Antioxidants (pharmacology)
• Apoptosis (drug effects)
• Brain (drug effects, metabolism, pathology)
• Brain Infarction (genetics, metabolism, pathology, prevention & control)
• Cyclohexane Monoterpenes (pharmacology)
• Cytokines (genetics, metabolism)
• Disease Models, Animal
• Inflammation Mediators (metabolism)
• Male
• NLR Family, Pyrin Domain-Containing 3 Protein (genetics, metabolism)
• Neuroprotective Agents (pharmacology)
• Oxidative Stress (drug effects)
• Rats, Wistar
• Reperfusion Injury (genetics, metabolism, pathology, prevention & control)
• Signal Transduction
• Toll-Like Receptor 4 (genetics, metabolism)