Identification and characterization of PEDV infection in rat crypt epithelial cells.

Abstract

Porcine epidemic diarrhea (PED) is a devastating enteric disease to the world's swine production. Porcine epidemic diarrhea virus (PEDV), as the PED causative agent, has been commonly propagated and investigated in Vero cells, as well as in IPEC-J2, a porcine epithelial cell-jejunum 2. However, Vero cells, which are defective in interferon production, cannot represent the host response in enteric cells while PEDV replicates poorly in IPEC-J2 cells. In this study, we observed that rat crypt epithelial cells (IEC-6) were highly susceptible to different subtypes of PEDV. The replication kinetics of PEDV in IEC-6 cells is similar to that in Vero cells, but it is much higher than in IPEC-J2 cells. Besides that, PEDV infection in IEC-6 cells can induce the production of inflammatory cytokines and interferon, especially the type III IFNs. Collectively, our findings suggest that IEC-6 is an ideal cell line for PEDV replication and immune response studies.

• Authors: Jianing Chen, Yaru Cui, Zemei Wang, Guangliang Liu
• Journal: Veterinary microbiology (Vet Microbiol) Vol. 249 Pg. 108848 (Oct 2020) ISSN: 1873-2542 [Electronic] Netherlands
• PMID: 32979749 (Publication Type: Journal Article)
• Copyright: Copyright © 2020 Elsevier B.V. All rights reserved.

Topics

• Animals
• Cell Line
• Coronavirus Infections (diagnosis, pathology, veterinary, virology)
• Epithelial Cells (virology)
• Porcine epidemic diarrhea virus (physiology)
• Rats