The immune function of heme oxygenase-1 from grass carp (Ctenopharyngodon idellus) in response to bacterial infection.

Abstract

Heme oxygenase (HO)-1, a rate-limiting enzyme in heme catabolism, results in the formation of equivalent amounts of biliverdin (BV), carbon monoxide (CO) and ferrous iron (Fe2+). Previous studies have revealed that HO-1 plays an important role in immune responses. However, the mechanism underlying the immune responses against bacterial infection of teleost HO-1 remains enigmatic. To decipher the mechanisms, we have cloned and characterized the HO-1 gene of grass carp (designated as GcHO-1) in this research. The results showed that the open reading frame (ORF) of GcHO-1 was 819 bp, which encoded a putative protein of 272 amino acids. The deduced amino acid sequence phylogenetically shared the highest identity with other teleosts, and contained two domains of heme-oxygenase and a single-pass transmembrane domain. The mRNA expressions of GcHO-1 in healthy grass carp have widely existed in examined tissues in the following order of spleen > head-kidney > middle head-kidney > intestines > liver > gills > heart > muscle > brain. Besides, the mRNA and protein transcription of GcHO-1 were both significantly up-regulated in the liver and head-kidney tissues after Staphylococcus aureus and Aeromonas hydrophila infection. In addition, overexpression of GcHO-1 in kidney cell line (CIK) cells of grass carp could reduce the expression of inflammatory cytokines (IL-1β, IL-8, TNFα, CCL1 and IL-6). Herein, we demonstrate that GcHO-1 plays an anti-inflammatory role in innate immunity. Our results shed new light on the mechanisms underlying the antibacterial immunity of teleost.

• Authors: Zhijie Lu, Fanbin Zhan, Minxuan Yang, Fenglin Li, Fei Shi, Yanan Li, Menglan Zhang, Lijuan Zhao, Kai Zhang, Jun Li, Li Lin, Zhendong Qin
• Journal: Fish & shellfish immunology (Fish Shellfish Immunol) Vol. 112 Pg. 168-178 (May 2021) ISSN: 1095-9947 [Electronic] England
• PMID: 32927052 (Publication Type: Journal Article)
• Copyright: Copyright © 2020 Elsevier Ltd. All rights reserved.

Chemical References

• Fish Proteins
• Heme Oxygenase-1

Topics

• Aeromonas hydrophila (physiology)
• Amino Acid Sequence
• Animals
• Base Sequence
• Carps (genetics, immunology)
• Fish Diseases (immunology, microbiology)
• Fish Proteins (chemistry, genetics, immunology)
• Gene Expression Profiling (veterinary)
• Gene Expression Regulation (immunology)
• Gram-Negative Bacterial Infections (immunology, veterinary)
• Heme Oxygenase-1 (chemistry, genetics, immunology)
• Immunity, Innate (genetics)
• Phylogeny
• Sequence Alignment (veterinary)
• Staphylococcal Infections (immunology, veterinary)
• Staphylococcus aureus (physiology)