Abstract | BACKGROUND: Chikungunya virus (CHIKV) is an arthropod-borne RNA virus which induces host Endoplasmic Reticulum (ER) stress by accumulating unfolded or misfolded proteins. ER stress activates the unfolded protein response (UPR) pathway to enable proper protein folding and maintain cellular homeostasis. There is no approved drug or vaccine available for CHIKV treatment, therefore, a pharmacological countermeasure is warranted for preventing CHIKV infection. OBJECTIVE: METHODS: Cells and supernatant collected on andrographolide and VER-155008, a GRP78 inhibitor, treatment in CHIKV infected and mock-infected THP-1 cells were tested for differential expression of UPR pathway proteins including GRP78, PERK, EIF-2α, IRE-1α, XBP-1 and ATF6. Furthermore, the inflammasome and apoptosis pathway proteins, i.e., caspase-1, caspase-3 and PARP, were tested by immunoblotting, and cytokines, i.e., IL-1β, IL-6 and IFN-γ were tested by ELISA. RESULTS: CONCLUSION: The study, therefore, confirms that andrographolide is a potential remedy for chikungunya infection and suppresses CHIKV induced ER stress and apoptosis.
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Authors | Swati Gupta, Kamla Prasad Mishra, Bhuvnesh Kumar, Shashi Bala Singh, Lilly Ganju |
Journal | Combinatorial chemistry & high throughput screening
(Comb Chem High Throughput Screen)
Vol. 24
Issue 6
Pg. 849-859
( 2021)
ISSN: 1875-5402 [Electronic] United Arab Emirates |
PMID | 32819227
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright© Bentham Science Publishers; For any queries, please email at [email protected]. |
Chemical References |
- Antiviral Agents
- Diterpenes
- Endoplasmic Reticulum Chaperone BiP
- HSPA5 protein, human
- andrographolide
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Topics |
- Antiviral Agents
(pharmacology)
- Apoptosis
(drug effects)
- Chikungunya Fever
(drug therapy, pathology)
- Chikungunya virus
(drug effects)
- Diterpenes
(pharmacology)
- Endoplasmic Reticulum Chaperone BiP
- Humans
- Microbial Sensitivity Tests
- THP-1 Cells
- Unfolded Protein Response
(drug effects)
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