Panax notoginseng saponin R1 modulates TNF-α/NF-κB signaling and attenuates allergic airway inflammation in asthma.

Abstract

BACKGROUD:

Panax notoginseng saponin R1 (PNS-R1) is one of the most important chemical monomers derived from the panax notoginseng, and our previous study found that PNS-R1 reduced glucocorticoid-induced apoptosis in asthmatic airway epithelial cells. Thus, in this study, we explored the effects of the PNS-R1 on inflammation of allergic asthma.

METHODS:

The asthmatic mice were administered 15 mg/kg PNS-R1 by intraperitoneal injection three days before sensitized to OVA. The effects of PNS-R1 on asthmatic mice were detected by airway hyperresponsiveness, inflammation, mucus hypersecretion and inflammatory cytokines such as interleukin (IL)-13, IL-4, IL-5, IL-8 and tumor necrosis factor (TNF)-α were studied. We also treated human bronchial epithelial cells (16HBE) with house dust mites (HDM) and then detected the secretion of cellular inflammatory factors (IL-13 and TNF-α). Western blot and immunofluorescence were used to examine the effect of PNS-R1 on TNF-α/NF-κB pathway. TNF-α/NF-κB/IKK signal pathway activator was used in PNS-R1-treated asthmatic mice.

RESULTS:

PNS-R1 significantly reduced the airway inflammatory, mucus secretion and hyperresponsiveness in asthma model. It also reduced the levels of IL-13, IL-4, IL-5 and IL-8 in bronchoalveolar lavage fluid (BALF) and IgE and OVA-specific IgE in serum for asthma mice. PNS-R1 reduced IL-13 and TNF-α secretion in HDM-treated 16HBE cells. In addition, PNS-R1 suppressed TNF-α/NF-κB pathway in both asthmatic mice and 16HBE. Activation of NF-kB pathway reversed the therapeutic effect of PNS-R1 on asthmatic mice.

CONCLUSION:

The results indicated that PNS-R1 effectively suppresses allergic airway inflammation of asthma partly through TNF-α/NF-κB pathway. PNS-R1 may play a potential role in allergic asthma treatment in the future.

• Authors: Kunjiao Xue, Lingying Ruan, Jie Hu, Zhou Fu, Daiyin Tian, Wenjing Zou
• Journal: International immunopharmacology (Int Immunopharmacol) Vol. 88 Pg. 106860 (Nov 2020) ISSN: 1878-1705 [Electronic] Netherlands
• PMID: 32771949 (Publication Type: Journal Article)
• Copyright: Copyright © 2020 Elsevier B.V. All rights reserved.

Chemical References

• Anti-Asthmatic Agents
• Muc5ac protein, mouse
• Mucin 5AC
• NF-kappa B
• Saponins
• Tumor Necrosis Factor-alpha
• Immunoglobulin E
• Ovalbumin
• I-kappa B Kinase

Topics

• Animals
• Anti-Asthmatic Agents (pharmacology, therapeutic use)
• Asthma (chemically induced, drug therapy)
• Bronchoalveolar Lavage Fluid (chemistry, immunology)
• Cell Line
• Disease Models, Animal
• Female
• Humans
• I-kappa B Kinase (metabolism)
• Immunoglobulin E (blood)
• Inflammation (chemically induced, drug therapy, metabolism)
• Lung (immunology, pathology)
• Male
• Mice, Inbred C57BL
• Mucin 5AC (drug effects, metabolism)
• Mucus (drug effects, metabolism)
• NF-kappa B (metabolism)
• Ovalbumin (toxicity)
• Panax notoginseng (chemistry)
• Pyroglyphidae
• Respiratory Hypersensitivity (chemically induced, drug therapy)
• Saponins (pharmacology, therapeutic use)
• Signal Transduction (drug effects)
• Tumor Necrosis Factor-alpha (metabolism)