Abstract |
The cytokine, GDF15, is produced in pathological states which cause cellular stress, including cancer. When over expressed, it causes dramatic weight reduction, suggesting a role in disease-related anorexia. Here, we demonstrate that the GDF15 receptor, GFRAL, is located in a subset of cholecystokinin neurons which span the area postrema and the nucleus of the tractus solitarius of the mouse. GDF15 activates GFRALAP/NTS neurons and supports conditioned taste and place aversions, while the anorexia it causes can be blocked by a monoclonal antibody directed at GFRAL or by disrupting CCK neuronal signalling. The cancer-therapeutic drug, cisplatin, induces the release of GDF15 and activates GFRALAP/NTS neurons, as well as causing significant reductions in food intake and body weight in mice. These metabolic effects of cisplatin are abolished by pre-treatment with the GFRAL monoclonal antibody. Our results suggest that GFRAL neutralising antibodies or antagonists may provide a co-treatment opportunity for patients undergoing chemotherapy.
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Authors | Amy A Worth, Rosemary Shoop, Katie Tye, Claire H Feetham, Giuseppe D'Agostino, Garron T Dodd, Frank Reimann, Fiona M Gribble, Emily C Beebe, James D Dunbar, Jesline T Alexander-Chacko, Dana K Sindelar, Tamer Coskun, Paul J Emmerson, Simon M Luckman |
Journal | eLife
(Elife)
Vol. 9
(07 29 2020)
ISSN: 2050-084X [Electronic] England |
PMID | 32723474
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2020, Worth et al. |
Chemical References |
- GDF15 protein, human
- Gdf15 protein, mouse
- Growth Differentiation Factor 15
- Recombinant Proteins
- Cholecystokinin
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Topics |
- Animals
- Anorexia
(genetics)
- Brain Stem
(physiology)
- Cholecystokinin
(metabolism)
- Growth Differentiation Factor 15
(administration & dosage, genetics, metabolism)
- Male
- Mice
- Mice, Inbred C57BL
- Neurons
(physiology)
- Pica
(genetics)
- Random Allocation
- Rats
- Rats, Sprague-Dawley
- Recombinant Proteins
(administration & dosage)
- Signal Transduction
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