Abstract | BACKGROUND: METHODS: Repetitive pulse stimulation with IFN-β1a (IS) was used to generate IS sublines that had acquired resistance to IFN-β-induced suppression of sphere formation. These cell lines were characterized by analyses of type 1 IFN signaling, growth patterns, and transcriptomic profiles. RESULTS: Here we report that repetitive IFN-β1a stimulation (IS) induces a stable phenotype (referred to as IS) at the level of maintaining sphere formation, although classical IFN signaling defined by the expression of both IFN receptors, myxovirus resistance protein A (MxA) accumulation, and STAT1 induction is unaffected. Furthermore, this stably altered IS phenotype is characterized by constitutively decreased sphere formation capacity and morphological features of senescence and autophagy. Transcriptional profiling reveals increased type I IFN signaling in these IS cells, but decreased expression of genes involved in receptor signaling and cell migration. CONCLUSIONS: Altogether, these data suggest a role for promoting IFN-β signaling in glioblastoma and might provide clues to design future therapeutic approaches.
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Authors | Birthe Lohmann, Manuela Silginer, Daniel Picard, Hannah Schneider, Marc Remke, Patrick Roth, Guido Reifenberger, Michael Weller |
Journal | Neuro-oncology advances
(Neurooncol Adv)
2020 Jan-Dec
Vol. 2
Issue 1
Pg. vdaa043
ISSN: 2632-2498 [Electronic] England |
PMID | 32642697
(Publication Type: Journal Article)
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Copyright | © The Author(s) 2020. Published by Oxford University Press, the Society for Neuro-Oncology and the European Association of Neuro-Oncology. |