[Effect of San'ao Decoction on ovalbum induced asthmatic mice and expression of TRPV2 in lung].

Abstract

To observe the efficacy of San'ao Decoction(SAD) in diffusing the lung and relieving asthma, and its intervention effect on the expression of transient receptor potential V2(TRPV2) during alleviating asthma, this study replicated an ovalbumin(OVA)-induced asthmatic mice model, and investigated the intervention effect of SAD on the airway inflammation and airway hyperresponsiveness. The regulatory mechanisms of SAD on the mRNA and protein expressions of TRPV2 in lung tissues and the levels of interleukin-4(IL-4),-10(IL-10), nerve growth factor(NGF), prostaglandin D_2(PGD_2) in bronchoalveolar lavage fluid(BALF) were discussed. Compared with the control group, the model group showed typical asthmatic phenotype, the level of eosinophils(EOS) in peripheral blood and BALF as well as the airway hyperresponsiveness were increased(P<0.01), and pathological damage in lung tissue was serious. The mRNA and protein expressions of TRPV2 in lung tissue were increased significantly, while the levels of IL-4, IL-10, NGF and PGD_2 in BALF were elevated(P<0.05,P<0.01). SAD could relieve bronchial asthma manifested as repaired lung patholo-gical changes(P<0.05), reduce the level of EOS in blood and BALF(P<0.05, P<0.01), and improve pulmonary resistance and lung compliance(P<0.05, P<0.01). SAD could also regulate the inflammatory cytokine levels of IL-4, IL-10, NGF, PGD_2 in BALF, and reduce the gene and protein expression of TRPV2 in the lung tissue(P<0.05, P<0.01). It is verified that SAD could reduce the lung inflammation, and improve lung function in asthmatic mice. The regulatory mechanism of SAD on asthma induced by OVA might be related to the regulation of TRPV2 expression and the induced decrease of Th2-related cytokines and neuropeptides, which provides the evidences for the treatment of asthma with SAD.

• Authors: Jing-Jing Zhang, Meng-Wen Li, Xin-Sheng Fan, Yue Zhu
• Journal: Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica (Zhongguo Zhong Yao Za Zhi) Vol. 45 Issue 11 Pg. 2619-2625 (Jun 2020) ISSN: 1001-5302 [Print] China
• PMID: 32627497 (Publication Type: Journal Article)

Chemical References

• Calcium Channels
• Drugs, Chinese Herbal
• San-Ao decoction
• TRPV Cation Channels
• Trpv2 protein, mouse
• Ovalbumin

Topics

• Animals
• Asthma
• Bronchoalveolar Lavage Fluid
• Calcium Channels
• Disease Models, Animal
• Drugs, Chinese Herbal
• Lung
• Mice
• Mice, Inbred BALB C
• Ovalbumin
• TRPV Cation Channels