MiR-155-5p plays as a "janus" in the expression of inflammatory cytokines induced by T-2 toxin.

Abstract

Although many studies have shown that inflammatory response plays a crucial role in the various toxic effects of T-2 toxin, there are relatively few reports on the mechanism of this phenomenon. Meanwhile, accumulating evidence has shown that miR-155-5p is activated in the inflammatory response. As molecular pathways and mechanisms involved in T-2 toxin-induced inflammatory response are poorly elucidated, we assessed whether miR-155-5p is involved in the inflammation effects mediated by T-2 toxin. Treatment of RAW264.7 cells with T-2 toxin (14 nM and 12 h) resulted in inflammatory response and associated with alteration of the gene expression signature of miR-155-5p. Knockdown or overexpression of miR-155-5p both indicated that miR-155-5p positively regulated the expression of the inflammation factors. Moreover, bioinformatics prediction and luciferase assay indicated that atg3 and rheb are targets of miR-155-5p. However, atg3 and SOCS1 play positive roles in the inflammatory response regulated by miR-155-5p, while rheb plays a negative role. In addition, the in vivo study showed that single administration of T-2 toxin in mice enhances spleen immune response, which was accompanied by an overexpression of miR-155-5p. These findings indicate that miR-155-5p might have an important role associated with the inflammatory response induced by T-2 toxin. In conclusion, a dual character of miR-155-5p in inflammation response was revealed, which might exist in other reactions in which miR-155-5p is involved.

• Authors: Pu Guo, Fang Qiao, Deyu Huang, Qinghua Wu, Tianlun Chen, Sara Badawy, Guyue Cheng, Haihong Hao, Shuyu Xie, Xu Wang
• Journal: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association (Food Chem Toxicol) Vol. 140 Pg. 111258 (Jun 2020) ISSN: 1873-6351 [Electronic] England
• PMID: 32240701 (Publication Type: Journal Article)
• Copyright: Copyright © 2020 Elsevier Ltd. All rights reserved.

Chemical References

• Cytokines
• Inflammation Mediators
• MicroRNAs
• Mirn155 microRNA, mouse
• T-2 Toxin

Topics

• Animals
• Cytokines (metabolism)
• Inflammation Mediators (metabolism)
• Mice
• MicroRNAs (metabolism)
• RAW 264.7 Cells
• Signal Transduction
• T-2 Toxin
• Up-Regulation