Abstract |
Tumour cell phagocytosis by antigen presenting cells (APCs) is critical to the generation of antitumour immunity. However, cancer cells can evade phagocytosis by upregulating anti-phagocytosis molecule CD47. Here, we show that CD47 blockade alone is inefficient in stimulating glioma cell phagocytosis. However, combining CD47 blockade with temozolomide results in a significant pro-phagocytosis effect due to the latter's ability to induce endoplasmic reticulum stress response. Increased tumour cell phagocytosis subsequently enhances antigen cross-presentation and activation of cyclic GMP-AMP synthase-stimulator of interferon genes (cGAS- STING) in APCs, resulting in more efficient T cell priming. This bridging of innate and adaptive responses inhibits glioma growth, but also activates immune checkpoint. Sequential administration of an anti-PD1 antibody overcomes this potential adaptive resistance. Together, these findings reveal a dynamic relationship between innate and adaptive immune regulation in tumours and support further investigation of phagocytosis modulation as a strategy to enhance cancer immunotherapy responses.
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Authors | Christina A von Roemeling, Yifan Wang, Yaqing Qie, Hengfeng Yuan, Hai Zhao, Xiujie Liu, Zhaogang Yang, Mingming Yang, Weiye Deng, Katelyn A Bruno, Charles K Chan, Andrew S Lee, Stephen S Rosenfeld, Kyuson Yun, Aaron J Johnson, Duane A Mitchell, Wen Jiang, Betty Y S Kim |
Journal | Nature communications
(Nat Commun)
Vol. 11
Issue 1
Pg. 1508
(03 20 2020)
ISSN: 2041-1723 [Electronic] England |
PMID | 32198351
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)
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Chemical References |
- CD47 Antigen
- CD47 protein, human
- Nucleotidyltransferases
- cGAS protein, human
- Temozolomide
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Topics |
- Adaptive Immunity
- Animals
- Antigen Presentation
- Apoptosis
- CD47 Antigen
(drug effects, metabolism)
- Cell Line, Tumor
- Cell Proliferation
- Disease Models, Animal
- Endoplasmic Reticulum
(metabolism)
- Glioblastoma
(immunology, pathology)
- Glioma
(immunology)
- Humans
- Immunity, Innate
- Immunotherapy
(methods)
- Mice
- Mice, Inbred C57BL
- Monitoring, Immunologic
- Nucleotidyltransferases
(metabolism)
- Phagocytosis
(immunology)
- T-Lymphocytes
(immunology)
- Temozolomide
(pharmacology)
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