Erythema nodosum leprosum (ENL) is an inflammatory complication in
leprosy. Yet, the involvement of ENL neutrophils in the inflammatory response against Mycobacterium leprae remains poorly explored. Our primary aim was to investigate the utility of the surface expression of neutrophil IL-10R1 as an ENL
biomarker and, secondarily, to evaluate whether
leprosy or healthy M. leprae-stimulated neutrophils produce
cytokines and are able to respond to
IL-10. We, in this study, describe a subpopulation of circulating neutrophils of ENL patients that exclusively expressed IL-10R1, providing evidence that IL-10R1+ neutrophils are present in ENL lesions. It was also found that ENL neutrophils, but not those of nonreactional
leprosy controls, were able to secret detectable levels of TNF ex vivo and the addition of
IL-10 blocked TNF release. It was likewise observed that M. leprae-stimulated, healthy neutrophils expressed IL-10R1 in vitro, and ENL-linked
cytokines were released by M. leprae-cultured neutrophils in vitro. Moreover, consistent with the presence of a fully functional IL-10R, the addition of
IL-10 prevented the release of M. leprae-induced
cytokines. Most importantly, dead M. leprae revealed its superior capacity to induce CCL4 and
IL-8 in primary neutrophils over live Mycobacterium, suggesting that M. leprae may hamper the inflammatory machinery as an immune escape mechanism.