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A membrane-tethering pepducin derived from formyl peptide receptor 3 shows strong therapeutic effects against sepsis.

Abstract
Formyl peptide receptors (FPRs) are G protein-coupled receptors mainly expressed in inflammatory myeloid cells. Previous reports demonstrated that human neutrophils express only FPR1 and FPR2 but not FPR3. Here, we found that FPR3 is expressed in sepsis patient derived neutrophils and Fpr3 is expressed in the mouse neutrophils. To test the role of Fpr3 in neutrophil activity, we synthesized Fpr3 pepducins and successfully developed an agonistic pepducin that stimulates Fpr3, eliciting calcium increase and chemotactic migration of neutrophils. We also found that administration of an Fpr3 pepducin in an experimental mouse sepsis model significantly increased the survival rate. The pepducin markedly inhibited lung injury, splenocyte apoptosis, and inflammatory cytokine production. Bacterial counts were significantly decreased by the pepducin in septic mice. Based on these results, we suggest that FPR3 can be regarded as a new target to control sepsis, and the newly generated Fpr3-based pepducin can be used for the development of anti-septic agents.
AuthorsHa Young Lee, Hyung Sik Kim, Yu Sun Jeong, Ji Cheol Kim, Yong-Soo Bae, You Hwan Jo, Joon Seong Park, Yoe-Sik Bae
JournalBiochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 524 Issue 1 Pg. 156-162 (03 26 2020) ISSN: 1090-2104 [Electronic] United States
PMID31982133 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2020 Elsevier Inc. All rights reserved.
Chemical References
  • Cytokines
  • Inflammation Mediators
  • Lipopeptides
  • Receptors, Formyl Peptide
Topics
  • Animals
  • Cecum (pathology)
  • Cell Membrane (drug effects, metabolism)
  • Cytokines (biosynthesis)
  • HEK293 Cells
  • Humans
  • Inflammation Mediators (metabolism)
  • Ligation
  • Lipopeptides (administration & dosage, pharmacology, therapeutic use)
  • Male
  • Mice, Inbred C57BL
  • Neutrophils (metabolism)
  • Punctures
  • Receptors, Formyl Peptide (metabolism)
  • Sepsis (drug therapy, pathology)

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