Transforming growth factor-beta1 promotes Geistlich Bio-Oss® osteogenesis via inhibiting local inflammation response in vivo.

Abstract

Inhibiting inflammation is helpful in relieving the absorption of alveolar bone and promoting periodontal bone regeneration. In a previous study, we showed that transforming growth factor-beta1 (TGF-β1)-induced Treg cells inhibit the absorption of tissue-engineered cartilage caused by endogenous IFN-γ and TNF-α. In this study, we investigated the effect of inhibiting local inflammatory responses on Geistlich Bio-Oss® osteogenesis promotion in vivo. TGF-β1+BMMSCs (bone marrow mesenchymal stem cells) were cultured in Geistlich Bio-Oss® medium, and biocompatibility was evaluated. Alveolar bone defects in New Zealand rabbits repaired by application of Geistlich Bio-Oss® were compared to the effects of added TGF-β1+BMMSCs. There was no significant difference between the untreated Geistlich Bio-Oss® medium-control group and the group treated with the addition of TGF-β1+BMMSCs. Pro-inflammatory cytokines IFN-γ and TNF-α delayed Geistlich Bio-Oss®-induced osteogenesis, but no significant difference in osteogenesis was seen with the addition of TGF-β1+BMMSCs. Geistlich Bio-Oss® has good compatibility with TGF-β1+BMMSCs. However, the dual role of in vivo TGF-β1+BMMSCs in regenerating periodontal bone and limiting local inflammation is not clear.

• Authors: Chichi Li, Wei Bi, Dan Zhang, Zhijian Sang, Liqun Li, Youcheng Yu
• Journal: International journal of clinical and experimental pathology (Int J Clin Exp Pathol) Vol. 10 Issue 9 Pg. 9310-9317 ( 2017) ISSN: 1936-2625 [Electronic] United States
• PMID: 31966803 (Publication Type: Journal Article)
• Copyright: IJCEP Copyright © 2017.