Abstract |
Baicalein (5,6,7-trihydroxyflavone), isolated from the root of traditional Chinese herb Scutellaria baicalensis Georgi, has anti-inflammatory and anti-oxidative activities. This study explored the protective and modulatory mechanisms of baicalein on neuroinflammation, oxidative stress and metabolic abnormality in lipopolysaccharide (LPS)-activated BV-2 cells. Our results demonstrated that treatment with baicalein remarkably restrained the production of pro-inflammatory factors including nitric oxide (NO), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in LPS-activated BV-2 cells. Moreover, baicalein significantly inhibited reactive oxygen species (ROS) production, decreased cyclooxygenase-2 (COX-2) and nuclear factor-b (NF-κB)/p65 expression. 1H NMR metabolomics analysis revealed that 12 differential metabolites were regulated by baicalein, implicated in alanine, aspartate and glutamate metabolism, glutathione metabolism, arginine and proline metabolism, D-glutamine and D-glutamate metabolism. In conclusion, these results indicated that baicalein has protective and modulatory effects on neuroinflammation and oxidative stress in LPS-activated BV-2 cells.
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Authors | Jiao-Jiao Yan, Guan-Hua Du, Xue-Mei Qin, Li Gao |
Journal | International immunopharmacology
(Int Immunopharmacol)
Vol. 79
Pg. 106092
(Feb 2020)
ISSN: 1878-1705 [Electronic] Netherlands |
PMID | 31863920
(Publication Type: Journal Article)
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Copyright | Copyright © 2019 Elsevier B.V. All rights reserved. |
Chemical References |
- Anti-Inflammatory Agents
- Cytokines
- Flavanones
- Inflammation Mediators
- Lipopolysaccharides
- NF-kappa B
- baicalein
- Ptgs2 protein, mouse
- Cyclooxygenase 2
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Topics |
- Animals
- Anti-Inflammatory Agents
(pharmacology)
- Cell Line
- Cyclooxygenase 2
(metabolism)
- Cytokines
(metabolism)
- Flavanones
(pharmacology)
- Gene Expression Regulation
- Inflammation Mediators
(metabolism)
- Lipopolysaccharides
(metabolism)
- Metabolic Diseases
(drug therapy)
- Mice
- Microglia
(metabolism, pathology)
- NF-kappa B
(metabolism)
- Neurogenic Inflammation
(drug therapy)
- Signal Transduction
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