Abstract | OBJECTIVE: It is well known that protein malnutrition (PM) states can affect hematopoiesis, leading to severe leukopenia and reduced number of granulocytes, which act as the first line of defense, and are important to the innate immune response. The aim of this study was to elucidate some of the mechanisms involved in the impairment of granulopoiesis in PM. METHODS: RESULTS: Malnourished animals presented with leukopenia associated with reduced number of granulocytes and reduced percentage of granulocytic progenitors; however, no differences were observed in the regulatory granulopoietic cytokine G-CSF. Additionally, the malnourished group presented with impaired response to in vivo G-CSF stimulus compared with control animals. PM was implicated in decreased ability of c-Kit+ cells to differentiate into myeloid progenitor cells and downregulated STAT3 signaling. Furthermore, the malnourished group exhibited reduced expression of G-CSF receptor on granule-monocytic progenitors. This reduced expression was not completely reversible with G-CSF treatment. CONCLUSIONS: This study implies that PM promotes intrinsic alterations to hematopoietic precursors, which result in hematologic changes, mainly neutropenia, observed in peripheral blood in PM states.
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Authors | Araceli Aparecida Hastreiter, Edson Naoto Makiyama, Primavera Borelli, Ricardo Ambrósio Fock |
Journal | Nutrition (Burbank, Los Angeles County, Calif.)
(Nutrition)
Vol. 69
Pg. 110540
(01 2020)
ISSN: 1873-1244 [Electronic] United States |
PMID | 31525700
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2019 Elsevier Inc. All rights reserved. |
Chemical References |
- Receptors, Granulocyte Colony-Stimulating Factor
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Topics |
- Animals
- Diet, Protein-Restricted
(adverse effects)
- Granulocyte Precursor Cells
(metabolism)
- Male
- Mice
- Mice, Inbred C57BL
- Neutropenia
(blood, etiology)
- Protein Deficiency
(blood, etiology)
- Receptors, Granulocyte Colony-Stimulating Factor
(blood)
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