Role of ferroptosis in the process of acute radiation-induced lung injury in mice.

Abstract

Radiation-induced lung injury (RILI) is one of the most common and fatal complications of thoracic radiotherapy. Cell death is the critical point in RILI. Ferroptosis is discovered recently as a new type of cell death which is different from other forms. Our research investigated the role of ferroptosis in the process of acute RILI in mice. The levels of ROS in lungs and the inflammatory cytokine levels (TNF-α, IL-6, IL-10, and TGF-β1) in serum decreased significantly post ferroptosis inhibitor treatment in acute RILI. Ferroptotic characteristic changes of mitochondria in acute RILI was observed by transmission electron microscopy (TEM). Treatment with ferroptosis inhibitor significantly alleviated radiation-induced histopathological changes in mice lungs. Glutathione peroxidase 4 (GPX4), the key maker of the ferroptosis, was down-regulated in RILI. In summary, we observed that ferroptosis played a crucial role in acute RILI, and the ROS induced by irradaition might be the original trigger of ferroptosis in acute RILI. At the same time, ferroptosis may also affect the levels of inflammatory cytokines in acute RILI.

• Authors: Xuan Li, Xibing Zhuang, Tiankui Qiao
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 519 Issue 2 Pg. 240-245 (11 05 2019) ISSN: 1090-2104 [Electronic] United States
• PMID: 31493867 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2019 The Authors. Published by Elsevier Inc. All rights reserved.

Chemical References

• Cytokines
• Reactive Oxygen Species

Topics

• Animals
• Cytokines (blood)
• Ferroptosis
• Infrared Rays
• Lung Injury (metabolism)
• Mice
• Mice, Inbred C57BL
• Radiation Injuries (metabolism)
• Reactive Oxygen Species (analysis, metabolism)