Targeted delivery of ibrutinib to tumor-associated macrophages by sialic acid-stearic acid conjugate modified nanocomplexes for cancer immunotherapy.

Abstract

Ibrutinib (IBR), an irreversible Bruton's tyrosine kinase (BTK) inhibitor, is expected to be a potent therapeutic modality, given that BTK is overexpressed in tumor-associated macrophages (TAMs) and participates in promoting tumor progression, angiogenesis, and immunosuppression. However, rapid clearance in vivo and low tumor accumulation have rendered effective uptake of IBR by TAMs challenge. Herein, we designed and synthesized a sialic acid (SA)-stearic acid conjugate modified on the surface of nanocomplexes to encapsulate IBR (SA/IBR/EPG) for targeted immunotherapy. Amphiphilic egg phosphatidylglycerol (EPG) structure and strong IBR-EPG interactions render these nanocomplexes high IBR loading capacity, prolonged blood circulation, and optimal particle sizes (∼30 nm), which can effectively deliver IBR to the tumor, followed by subsequent internalization of IBR by TAMs through SA-mediated active targeting. In vitro and in vivo tests showed that the prepared SA/IBR/EPG nanocomplexes could preferentially accumulate in TAMs and exert potent antitumor activity. Immunofluorescence staining analysis further confirmed that SA/IBR/EPG remarkably inhibited angiogenesis and tumorigenic cytokines released by TAM and eventually suppressed tumor progression, without eliciting any unwanted effect. Thus, SA-decorated IBR nanocomplexes present a promising strategy for cancer immunotherapy. STATEMENT OF SIGNIFICANCE: Ibrutinib (IBR), an irreversible Bruton's tyrosine kinase (BTK) inhibitor, is expected to be a potent therapeutic modality, given that BTK is overexpressed in tumor-associated macrophages (TAMs) and participates in promoting tumor progression, angiogenesis, and immunosuppression. However, rapid clearance in vivo and low tumor accumulation have rendered effective uptake of IBR by TAMs challenge. Herein, we designed and synthesized a sialic acid (SA)-stearic acid conjugate modified on the surface of nanocomplexes to encapsulate IBR (SA/IBR/EPG) for targeted delivery of IBR to TAMs. The developed SA/IBR/EPG nanocomplexes exhibited high efficiency in targeting TAMs and inhibiting BTK activation, consequently inhibiting Th2 tumorigenic cytokine release, reducing angiogenesis, and suppressing tumor growth. These results implied that the SA/IBR/EPG nanocomplex could be a promising strategy for TAM-targeting immunotherapy with minimal systemic side effects.

• Authors: Qiujun Qiu, Cong Li, Yanzhi Song, Tao Shi, Xiang Luo, Hongxia Zhang, Ling Hu, Xinyang Yan, Huangliang Zheng, Mengyang Liu, Mingqi Liu, Min Liu, Shuaishuai Yang, Xinrong Liu, Guoliang Chen, Yihui Deng
• Journal: Acta biomaterialia (Acta Biomater) Vol. 92 Pg. 184-195 (07 01 2019) ISSN: 1878-7568 [Electronic] England
• PMID: 31108259 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2019 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

Chemical References

• Phosphatidylglycerols
• Piperidines
• Pyrazoles
• Pyrimidines
• Stearic Acids
• ibrutinib
• stearic acid
• N-Acetylneuraminic Acid
• Adenine

Topics

• Adenine (analogs & derivatives)
• Animals
• Drug Delivery Systems
• Immunotherapy
• Macrophages (drug effects, metabolism)
• Male
• Mice
• N-Acetylneuraminic Acid (chemical synthesis, chemistry)
• Nanoparticles (chemistry, ultrastructure)
• Neoplasms (drug therapy, immunology)
• Phosphatidylglycerols (chemistry)
• Piperidines
• Proton Magnetic Resonance Spectroscopy
• Pyrazoles (pharmacology, therapeutic use)
• Pyrimidines (pharmacology, therapeutic use)
• RAW 264.7 Cells
• Rats, Wistar
• Stearic Acids (chemical synthesis, chemistry)