We are developing an associated
vaccine based on live
influenza vaccine (LAIV) and streptococcal recombinant
peptides. The recombinant group B streptococcus (GBS)
peptides P6 and ScaAB demonstrated a distinguished immunomodulating effect in THP-1 cells. The increase in IFN 1-alpha expression after ScaAB inoculation was similar to that against LAIV. We immunized mice intranasal using of A/H7N3 LAIV or/and ScaAB
peptide. At day 5 after immunization, we detected serum
IgM which reacted with non-
vaccine influenza viruses. Associated vaccination of mice using LAIV and GBS
peptide was the most effective against sub-lethal
infection with A/H7N9 influenza virus and against lethal challenge with A/H1N1pdm virus at day 5 after immunization. Not only LAIV but also the ScaAB protected about 20% of the immunized animals against lethal challenge with A/H1N1pdm virus. The early protection was related to increasing type 1
interferons expression in the lungs. Our results in mice have shown that successful protection against homologous and heterologous
influenza infections can be achieved soon after vaccination with either LAIV or LAIV in combination with GBS recombinant
peptide. Presumably, such protection may be mediated by non-specific
IgM antibodies and an increase in the expression of early
cytokines in the airway.