Abstract | CONTEXT: OBJECTIVE: To determine whether chronic endogenous GC exposure due to CD is associated with adipose tissue (AT) inflammation in humans. DESIGN, SETTING, PARTICIPANTS: Abdominal subcutaneous AT samples from 10 patients with active CD and 10 age-, sex-, and body mass index‒matched healthy subjects were assessed for macrophage infiltration and mRNA expression of proinflammatory cytokines. MAIN OUTCOME MEASURE: Using immunohistochemistry, AT samples were analyzed for the expression of vimentin, caspase, CD3, CD4, CD8, CD11c, CD20, CD31, CD56, CD68, and CD163. Quantitative PCR was used to assess the mRNA gene expression of arginase, CD11b, CD68, EMR-1, IL-6, IL-10, MCP-1, and TNF-α. RESULTS: Immunohistochemistry revealed higher mean percentage infiltration of CD68+ macrophages and CD4+ T lymphocytes, increased mean area of CD11c+ M1 macrophages, higher number of CD11c+ crownlike structures, and decreased vimentin in the AT of patients with active CD compared with controls. PCR revealed no differences in mRNA expression of any analyzed markers in patients with CD. CONCLUSIONS:
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Authors | Irene T Lee, Alexandria Atuahene, Hale Ergin Egritag, Ling Wang, Michael Donovan, Christoph Buettner, Eliza B Geer |
Journal | The Journal of clinical endocrinology and metabolism
(J Clin Endocrinol Metab)
Vol. 104
Issue 6
Pg. 2453-2461
(06 01 2019)
ISSN: 1945-7197 [Electronic] United States |
PMID | 30722035
(Publication Type: Journal Article, Research Support, N.I.H., Extramural)
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Copyright | Copyright © 2019 Endocrine Society. |
Chemical References |
- Antigens, CD
- Antigens, Differentiation, Myelomonocytic
- CD11c Antigen
- CD68 antigen, human
- Cytokines
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Topics |
- Adipose Tissue
(pathology)
- Adult
- Aged
- Antigens, CD
(analysis)
- Antigens, Differentiation, Myelomonocytic
(analysis)
- CD11c Antigen
(analysis)
- Cytokines
(genetics)
- Female
- Humans
- Immunohistochemistry
- Inflammation
(etiology)
- Macrophages
(immunology)
- Male
- Middle Aged
- Pituitary ACTH Hypersecretion
(pathology)
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