Abstract |
Nontoxic and nonimmunogenic nanoparticles play an increasingly important role in the application of pharmaceutical nanocarriers. The pathogenesis of diabetic peripheral neuropathy ( DPN) has been extensively studied. However, the role of microRNAs in DPN remains to be clarified. We verified in vitro that miR-146a-5p mimics inhibited the expression of proinflammatory cytokines and apoptosis. Then, we explored the protective effect of nanoparticle-miRNA-146a-5p polyplexes (nano-miR-146a-5p) on DPN rats. We demonstrated that nano-miR-146a-5p improved nerve conduction velocity and alleviated the morphological damage and demyelination of the sciatic nerve of DPN rats. The expression of the inflammatory cytokines, caspase-3, and cleaved caspase-3 in the sciatic nerve was inhibited by nano-miR-146a-5p. Additionally, nano-miR-146a-5p increased the expression of myelin basic protein. These results all indicated that nano-miR-146a-5p had a protective effect on peripheral nerves in the DPN rat model, which may occur through the regulation of the inflammatory response and apoptosis.
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Authors | Qiong Luo, Yonghao Feng, Yangmei Xie, Yiye Shao, Men Wu, Xiaolin Deng, Wei-En Yuan, Yinghui Chen, Xiaohong Shi |
Journal | Nanomedicine : nanotechnology, biology, and medicine
(Nanomedicine)
Vol. 17
Pg. 188-197
(04 2019)
ISSN: 1549-9642 [Electronic] United States |
PMID | 30721753
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2019 The Author(s). Published by Elsevier Inc. All rights reserved. |
Chemical References |
- MIRN146a microRNA, rat
- MicroRNAs
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Topics |
- Animals
- Apoptosis
- Cell Line
- Diabetes Mellitus, Experimental
(complications, genetics, pathology)
- Diabetic Neuropathies
(genetics, pathology, therapy)
- Genetic Therapy
- Inflammation
(therapy)
- MicroRNAs
(therapeutic use)
- Nanoparticles
(therapeutic use)
- Rats
- Sciatic Nerve
(metabolism, pathology)
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