Abstract | BACKGROUND: Clinical, virologic, and immunologic characteristics of Zika virus (ZIKV) infections in US patients are poorly defined. METHODS: US subjects with suspected ZIKV infection were enrolled. Clinical data and specimens were prospectively collected for ZIKV RNA detection and serologic and cellular assays. Confirmed ZIKV infection (cases) and ZIKV-negative (controls) subjects were compared. Dengue-experienced and dengue-naïve cases were also compared. RESULTS: We enrolled 45 cases and 14 controls. Commonly reported symptoms among cases and controls were maculopapular rash (97.8% and 81.8%), fatigue (86.7% and 81.8%), and arthralgia (82.2% and 54.5%), respectively. The sensitivity (94%) and duration of infection detection (80% positivity at 65-79 days after disease onset) by polymerase chain reaction were highest in whole-blood specimens. ZIKV- neutralizing antibodies had a half-life of 105 days and were significantly higher in dengue virus-experienced cases than naïve ones (P = .046). In intracellular cytokine staining assays, the ZIKV proteins targeted most often by peripheral blood mononuclear cells from cases were structural proteins C and E for CD4+ T cells and nonstructural proteins NS3, NS5, and NS4B for CD8+ T cells. CONCLUSIONS:
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Authors | Hana M El Sahly, Rodion Gorchakov, Lilin Lai, Muktha S Natrajan, Shital M Patel, Robert L Atmar, Wendy A Keitel, Daniel F Hoft, Jill Barrett, Jason Bailey, Srilatha Edupuganti, Vanessa Raabe, Henry M Wu, Jessica Fairley, Nadine Rouphael, Kristy O Murray, Mark J Mulligan |
Journal | Open forum infectious diseases
(Open Forum Infect Dis)
Vol. 6
Issue 1
Pg. ofy352
(Jan 2019)
ISSN: 2328-8957 [Print] United States |
PMID | 30697574
(Publication Type: Journal Article)
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