Complement regulatory protein CD59a plays a protective role in immune liver injury of trichloroethylene-sensitized BALB/c mice.

Abstract

Trichloroethylene (TCE) is a major occupational and environmental chemical compound which causes occupational dermatitis medicamentosa-like of TCE with severe liver damage. Our previous studies showed that complement activation was a newly recognized mechanism for TCE-induced liver damage. The objective of this study was to explore the role of the key complement regulatory protein, CD59a, in TCE-induced immune liver injury. We firstly evaluated the changes of CD59a expression in liver tissue and then investigated if the changes were associated with membrane attack complex (MAC) formation, nuclear factor kappa B (NF-κB) activation and liver damage in BALB/c mice model of TCE-induced skin sensitization in the absence or presence of soluble recombinant rat CD59-Cys. The results showed that low expression of CD59a accompanied by MAC deposition in the liver of TCE-sensitized BALB/c mice, which was consistent in time. In addition, activation of NF-κB pathway, upregulation of inflammatory cytokine and liver damage also occured. Additional experiment showed that recombinant rat sCD59-Cys alleviated inflammation and liver damage in TCE-sensitized BALB/c mice. Moreover, recombinant rat sCD59-Cys reduced MAC formation and inhibited NF-κB activation measured by P-IκBα and nuclear NF-κB p65 in the liver of TCE-sensitized BALB/c mice. In conclusion, recombinant rat sCD59-Cys plays a protective role in immune liver injury of TCE-sensitized BALB/c mice.

• Authors: Xian Wang, Yun Yu, Hai-Bo Xie, Tong Shen, Qi-Xing Zhu
• Journal: Ecotoxicology and environmental safety (Ecotoxicol Environ Saf) Vol. 172 Pg. 105-113 (May 15 2019) ISSN: 1090-2414 [Electronic] Netherlands
• PMID: 30685621 (Publication Type: Journal Article)
• Copyright: Copyright © 2019 Elsevier Inc. All rights reserved.

Chemical References

• CD59 Antigens
• CD59a protein, mouse
• Complement Membrane Attack Complex
• NF-kappa B
• Recombinant Proteins
• Trichloroethylene

Topics

• Animals
• CD59 Antigens (genetics, metabolism)
• Chemical and Drug Induced Liver Injury (etiology, prevention & control)
• Complement Membrane Attack Complex (genetics, metabolism)
• Female
• Gene Expression Regulation
• Immunohistochemistry
• Inflammation (chemically induced, prevention & control)
• Mice
• Mice, Inbred BALB C
• NF-kappa B (genetics, metabolism)
• Recombinant Proteins (genetics, metabolism)
• Skin (drug effects, metabolism)
• Trichloroethylene (toxicity)