Abstract | BACKGROUND: In an increasing search for natural products that may heal the ulcers and avoid its recurrence, limonene appears as a promising candidate. HYPOTHESIS/PURPOSE: The present study aimed to investigate the protective effect of limonene in ethanol-induced gastric ulcers, in addition, to investigate the involvement of antioxidant and anti-inflammatory activities, besides the modulation of gene expression. STUDY DESIGN: METHODS: RESULTS: Our results showed that limonene 50 mg/kg was the lowest effective dose, offering 93% of reduction in gastric ulcer area compared with the vehicle. There was an increase in mucus production and higher preservation of gastric mucosa integrity after treatment with limonene.There was a reduction in the MPO activity, a biomarker of neutrophils infiltration, and an increase in GPx activity, suggesting an antioxidant effect. Limonene displayed anti-inflammatory activity through decreasing the levels of TNF-a, IL-6, and IL-1β and increasing the level of IL-10. Limonene could down-regulate the expression of Nf-κb, Il-1β, and Mpo and up-regulate the expression of Gpx. CONCLUSION: Our results demonstrate that oral treatment with limonene exerts gastroprotection through local mucosal defense mechanisms, such as increasing the mucus production, modulation of the oxidative stress and inflammatory response and inhibition of Nf-κb expression.
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Authors | Matheus Chiaradia de Souza, Ana Júlia Vieira, Fernando Pereira Beserra, Cláudia Helena Pellizzon, Rafael Henrique Nóbrega, Ariane Leite Rozza |
Journal | Phytomedicine : international journal of phytotherapy and phytopharmacology
(Phytomedicine)
Vol. 53
Pg. 37-42
(Feb 2019)
ISSN: 1618-095X [Electronic] Germany |
PMID | 30668410
(Publication Type: Journal Article)
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Copyright | Copyright © 2018 Elsevier GmbH. All rights reserved. |
Chemical References |
- Anti-Inflammatory Agents, Non-Steroidal
- Antioxidants
- Cytokines
- Protective Agents
- Ethanol
- Limonene
- Peroxidase
- Superoxide Dismutase
- Glutathione
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Topics |
- Administration, Oral
- Animals
- Anti-Inflammatory Agents, Non-Steroidal
(pharmacology)
- Antioxidants
(pharmacology)
- Cytokines
(metabolism)
- Dose-Response Relationship, Drug
- Ethanol
(adverse effects)
- Gastric Mucosa
(drug effects, pathology)
- Gastritis
(chemically induced, drug therapy)
- Gene Expression Regulation
(drug effects)
- Glutathione
(metabolism)
- Limonene
(administration & dosage, pharmacology)
- Male
- Oxidative Stress
(drug effects)
- Peroxidase
(metabolism)
- Protective Agents
(pharmacology)
- Rats, Wistar
- Stomach Ulcer
(chemically induced, genetics, prevention & control)
- Superoxide Dismutase
(metabolism)
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