Astaxanthin protects lipopolysaccharide-induced inflammatory response in Channa argus through inhibiting NF-κB and MAPKs signaling pathways.

Abstract

The present study was conducted to evaluate the protective effects of astaxanthin against lipopolysaccharide (LPS)-induced inflammatory responses in Channa argus in vivo and ex vivo. Primary hepatocytes were exposed to different concentrations of LPS for 24 h to induce an inflammatory response, and the protective effects of astaxanthin against LPS-induced inflammation were studied ex vivo and in vivo. Hepatocytes exposed to LPS (5-20 μg mL-1) alone for 24 h resulted in a significant increase in lactate dehydrogenase release (LDH), Nitric oxide (NO) production and Malondialdehyde (MDA) content, 10 μg mL-1 LPS could induced inflammatory response in hepatocytes. Gene expression of TLR4, NFkBp65, MAPKp38, TNF-α, IL-6 and IL-1β mRNA expression were also enhanced ex vivo (p < 0.05). In vivo test demonstrated that pretreatment with astaxanthin prevented the LPS-induced upregulation of pro-inflammatory cytokines TNF-α, IL-6 and IL-1β. Besides, astaxanthin blocked the expression of Toll-like receptor 4 (TLR4) and then suppressed the phosphorylation of nuclear transcription factor-kappa B (NF-κB) p65 and degradation inhibitor of NF-κBα (IκBα). Further study showed that astaxanthin could suppress the phosphorylation of p38, extracellular signal-regulated kinase (ERK) and c-jun NH2-terminal kinase (JNK) in mitogen-activated protein kinase (MAPK) signal pathway. In conclusion, our results suggest that astaxanthin played an anti-inflammatory role by regulating TLR4 and the NF-κB and MAPK signaling pathways in C. argus.

• Authors: Mu-Yang Li, Li Sun, Xiao-Tian Niu, Xiu-Mei Chen, Jia-Xin Tian, Yi-Di Kong, Gui-Qin Wang
• Journal: Fish & shellfish immunology (Fish Shellfish Immunol) Vol. 86 Pg. 280-286 (Mar 2019) ISSN: 1095-9947 [Electronic] England
• PMID: 30448447 (Publication Type: Journal Article)
• Copyright: Copyright © 2018 Elsevier Ltd. All rights reserved.

Chemical References

• Anti-Inflammatory Agents
• Lipopolysaccharides
• NF-kappa B
• Xanthophylls
• astaxanthine

Topics

• Animals
• Anti-Inflammatory Agents (pharmacology)
• Cells, Cultured
• Fishes
• Hepatocytes
• Inflammation (chemically induced, drug therapy)
• Lipopolysaccharides (pharmacology)
• MAP Kinase Signaling System (drug effects)
• NF-kappa B (drug effects)
• Signal Transduction
• Xanthophylls (pharmacology)