Salvianolic Acid B-Alleviated Angiotensin II Induces Cardiac Fibrosis by Suppressing NF-κB Pathway In Vitro.

Abstract

BACKGROUND Salvianolic acid B (SalB) is the representative component of phenolic acids derived from the roots and rhizomes of Salvia miltiorrhiza Bge (Labiatae), which has been used widely in Asian countries for clinical therapy of various cardiovascular dysfunction-related diseases. However, cardiac protection effects and the underlying mechanism for clinical application are still poorly understood. Here, we investigated the potential anti-myocardial fibrosis effect and mechanism of SalB on Angiotensin II (Ang II)-induced cardiac fibrosis in vitro. MATERIAL AND METHODS The proliferation and migration capacity of cardiac fibroblasts (CFBs) were measured by MTT assay and scratch analysis, respectively. The colorimetric assay determined the hydroxyproline content in medium. Western blotting detected the protein expressions of nuclear transcription factor-kappa B (NF-κB) pathway-associated proteins, fibronectin (FN), collagen type I (Coll I), α-smooth muscle actin (α-SMA), and connective tissue growth factor (CTGF). The expression of α-SMA protein was observed by immunofluorescence staining. qRT-PCR detected the mRNA expression of NF-κB. RESULTS SalB attenuated Ang II-induced the proliferation and the migration ability of CFBs. Ang II-induced the extracellular matrix protein Coll I, FN, and α-SMA, the pro-fibrotic cytokine CTGF protein expression was inhibited, and the nuclear translocation of NF-κB p65 subunit was reduced by SalB. Western blotting and qRT-PCR confirmed that SalB blocked the activation of NF-κB induced by Ang II. PDTC (the NF-κB inhibitor) also inhibited proliferation of CFBs and reduced α-SMA and Coll I expression induced by Ang II. CONCLUSIONS SalB can alleviate Ang II-induced cardiac fibrosis via suppressing the NF-κB pathway in vitro.

• Authors: Chunhua Wang, Hong Luo, Yini Xu, Ling Tao, Churui Chang, Xiangchun Shen
• Journal: Medical science monitor : international medical journal of experimental and clinical research (Med Sci Monit) Vol. 24 Pg. 7654-7664 (Oct 26 2018) ISSN: 1643-3750 [Electronic] United States
• PMID: 30365482 (Publication Type: Journal Article)

Chemical References

• Benzofurans
• Collagen Type I
• I-kappa B Proteins
• NF-kappa B
• Angiotensin II
• salvianolic acid B

Topics

• Angiotensin II (metabolism)
• Animals
• Benzofurans (metabolism, pharmacology)
• Cell Movement (drug effects)
• Cell Proliferation (drug effects)
• Collagen Type I (metabolism)
• Fibroblasts (drug effects)
• Fibrosis (drug therapy)
• Heart (drug effects)
• I-kappa B Proteins (metabolism)
• Myocardium (metabolism, pathology)
• NF-kappa B (metabolism)
• Rats
• Rats, Sprague-Dawley