Abstract |
Lymphocytes from peripheral blood (PB) and synovial fluid (SF) from 21 patients with rheumatoid arthritis (RA) and 18 patients with juvenile rheumatoid arthritis (JRA) were studied with respect to T cell phenotypes using monoclonal antibodies in a rosette assay. The percentage of HLA-DR positive T cells was counted in PB and SF using indirect immunofluorescence. Suppressor cell activity of T cells from PB and SF was investigated by measuring the immunoglobulin production by pokeweed mitogen (PWM) stimulated B cells mixed with T cells at various ratios. The mean T4/T8 ratio was significantly lower in SF than in PB of both RA and JRA patients (p = 0.0062 and p less than 0.0001 respectively). The mean percentages of HLA-DR positive T cells were elevated in SF compared with PB in both patients groups (p less than 0.03 and p less than 0.04 in RA and JRA patients respectively). Mean suppressor cell activity and helper cell activity of T cells from SF and PB of JRA patients was normal. Thus there seems to be a dichotomy between the number of T8+ cells and suppressor cell function in mononuclear cells from SF of patients with JRA. This indicates that a considerable proportion of the T8+ cells in the SF do not have suppressor functions.
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Authors | J Thoen, O Førre, K Waalen, E Kåss |
Journal | Scandinavian journal of rheumatology
(Scand J Rheumatol)
Vol. 16
Issue 4
Pg. 247-56
( 1987)
ISSN: 0300-9742 [Print] England |
PMID | 2957788
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
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Topics |
- Adolescent
- Adult
- Aged
- Arthritis, Juvenile
(blood)
- Arthritis, Rheumatoid
(blood)
- Child
- Female
- HLA-DR Antigens
(genetics)
- Humans
- Male
- Middle Aged
- Phenotype
- Synovial Fluid
(cytology)
- T-Lymphocytes
(immunology)
- T-Lymphocytes, Helper-Inducer
(immunology)
- T-Lymphocytes, Regulatory
(immunology)
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