Abstract | INTRODUCTION: METHODS: Male Wistar rats were randomly divided into 4 groups (control, control + TXR [150 mg/kg, daily], diabetic, and diabetic + TXR). Type-1 diabetes was induced by an intraperitoneal injection of streptozotocin (50 mg/kg) and lasted for 10 weeks. After mounting on the Langendorff apparatus, isolated hearts in all groups received a normal Krebs-Henseleit solution for 20 min of stabilization period, followed by 30 min of regional ischemia through ligation of the left anterior descending coronary artery, and 60 min of full reperfusion. During the experiment, the electrocardiograms were recorded and the arrhythmias [number, duration and incidence of premature ventricular complexes ( PVC), ventricular tachycardia (VT), ventricular fibrillation (VF), and arrhythmia score] during I/R phases were assessed based on the Lambeth Convention. Ischemic left ventricular samples were used to determine the activities of lactate dehydrogenase (LDH), interleukin-1beta (IL-1β), and tumor necrosis factor (TNF-α). RESULTS: The arrhythmias induced by I/R were not significantly changed in diabetic group as compared to the control group. However, pretreatment with TXR significantly reduced the number of PVC and duration and incidence of VF in ischemic phase in comparison to the untreated animals (P < 0.05). In addition, the duration, and incidence of most arrhythmias during reperfusion phase were significantly declined by TXR administration in both control and diabetic groups (P < 0.05). Pretreatment of rats with TXR significantly reduced myocardial inflammatory cytokines TNF-α and IL-1β levels after I/R insult in diabetic as well as control hearts (P < 0.05). CONCLUSION:
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Authors | Moslem Najafi, Elham Noroozi, Aniseh Javadi, Reza Badalzadeh |
Journal | Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
(Biomed Pharmacother)
Vol. 102
Pg. 385-391
(Jun 2018)
ISSN: 1950-6007 [Electronic] France |
PMID | 29573617
(Publication Type: Journal Article)
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Copyright | Copyright © 2018 Elsevier Masson SAS. All rights reserved. |
Chemical References |
- Anti-Arrhythmia Agents
- Anti-Inflammatory Agents
- Blood Glucose
- Cytokines
- Hydroxyethylrutoside
- troxerutin
- L-Lactate Dehydrogenase
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Topics |
- Animals
- Anti-Arrhythmia Agents
(pharmacology, therapeutic use)
- Anti-Inflammatory Agents
(pharmacology, therapeutic use)
- Blood Glucose
(metabolism)
- Body Weight
(drug effects)
- Cytokines
(metabolism)
- Diabetes Mellitus, Experimental
(blood, complications, diagnostic imaging, drug therapy)
- Electrocardiography
- Hydroxyethylrutoside
(analogs & derivatives, pharmacology, therapeutic use)
- L-Lactate Dehydrogenase
(metabolism)
- Male
- Myocardial Reperfusion Injury
(blood, complications, diagnostic imaging, drug therapy)
- Myocardium
(pathology)
- Rats, Wistar
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