Hepatic
fibrosis is the only chronic
liver disease process that can be reversed. Developing non-invasive and effective methods to quantitatively assess the degree of
liver fibrosis is of great clinical significance and remains a major challenge. The key factors in hepatic
fibrosis pathogenesis are the activation and proliferation of hepatic stellate cells that subsequently express
integrin αvβ3. An ultrasound (US) agent combined with a targeting
peptide may be used for the early and non-invasive diagnosis of hepatic
fibrosis. Herein, we report the synthesis of core-shell nanoparticles (NPs) successfully engineered by conjugation with cyclic
arginine-glycine-aspartic acid (cRGD) octapeptide, allowing hepatic
integrin αvβ3 targeting for
liver fibrosis staging. This system consists of a
perfluorooctyl bromide (
PFOB) liquid in the core that is stabilized with a
Poly (lactic-co-glycolic acid) (PLGA)
polymer shell and modified with a cRGD. These core-shell NPs (cRGD-PLGA-
PFOB NPs) exhibited useful US molecular imaging features including high imaging contrast among liver fibrotic stages and the adjacent tissues. Our results indicate that the cRGD-PLGA-
PFOB NPs have significant potential to distinguish different liver fibrotic stages and could be used in clinical applications.