[Expression of SOCS-1 and SOCS-3 in chronic periapical lesions and its clinical significance].

Abstract

PURPOSE:

To detect the expression of suppressor of cytokine signaling-1 (SOCS-1) and suppressor of cytokine signaling-3 (SOCS-3) in chronic periapical lesions and to clarify their roles in pathogenesis of apical periodontitis.

METHODS:

A total of 25 chronic periapical lesion tissues and 16 normal periodontal ligament tissues were collected respectively. The expression of mRNA was measured by real-time PCR, the protein expression was measured by immunohistochemical analysis. Statistical analysis was performed using SPSS 13.0 software package.

RESULTS:

Immunohistochemical results indicated that expression levels of SOCS-1 and SOCS-3 protein in chronic periapical lesions were significantly higher than that in normal tissues (P<0.01); Moreover, SOCS-1 and SOCS-3 protein expression levels in severe inflammation group were significantly higher than that in mild inflammation group (P<0.01). In mild inflammation group, severe inflammation group and control group, the expression levels of SOCS-1 mRNA were 2.620±1.552, 2.373±1.083 and 1.277±1.040, whereas those of SOCS-3 were 9.308±5.901, 7.565±3.233 and 1.232±1.099, respectively.

CONCLUSIONS:

The expression levels of SOCS-1 mRNA and SOCS-3 mRNA are significantly higher both in mild inflammation group and severe inflammation group than that in control group (P<0.01), while no significant difference is found in mild and severe inflammation group.

• Authors: Si-Mo Wang, Nan Ma, Li-Hong Qiu, Xiao-Lin Li, Di Yang, Ming Xue
• Journal: Shanghai kou qiang yi xue = Shanghai journal of stomatology (Shanghai Kou Qiang Yi Xue) Vol. 26 Issue 4 Pg. 384-388 (Aug 2017) ISSN: 1006-7248 [Print] China
• PMID: 29199331 (Publication Type: Journal Article)

Chemical References

• RNA, Messenger
• SOCS1 protein, human
• SOCS3 protein, human
• Suppressor of Cytokine Signaling 1 Protein
• Suppressor of Cytokine Signaling 3 Protein
• Suppressor of Cytokine Signaling Proteins

Topics

• Humans
• Inflammation
• Periapical Periodontitis (diagnosis, genetics, metabolism)
• RNA, Messenger
• Real-Time Polymerase Chain Reaction
• Suppressor of Cytokine Signaling 1 Protein (metabolism)
• Suppressor of Cytokine Signaling 3 Protein (metabolism)
• Suppressor of Cytokine Signaling Proteins