Abstract | OBJECTIVE: METHODS: LPG molecule was isolated and purified from large-scale Leishmania donovani parasite culture. Protection efficacies of LPG alone, in combination with Freund's adjuvant, in a physical mixture and in conjugate (consisting of various LPG concentrations) with PAA, were comparatively determined by various techniques, such as cultivation with the micro-culture method, assessment of in vitro infection rates of peritoneal macrophages, determination of parasite load in liver with Leishman-Donovan Units, and detection of cytokine responses. RESULTS: Obtained results demonstrated that the highest vaccine-mediated immune protection was provided by LPG-PAA conjugate due to all parameters investigated. According to the Leishman-Donovan Units results, the sharpest decline in parasite load was seen with a ratio of 81.17% when 35 μg LPG containing conjugate was applied. This value was 44.93% for the control group immunized only with LPG. Moreover, decreases in parasite load were 53.37%, 55.2% and 65.8% for the groups immunized with 10 μg LPG containing LPG-PAA conjugate, a physical mixture of the LPG-PAA, and a mixture of LPG + Freund's adjuvant, respectively. Furthermore, cytokine results supported that Th1 mediated protection occurred when mice were immunized with LPG-PAA conjugate. CONCLUSIONS:
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Authors | Adil M Allahverdiyev, Rabia Cakir Koc, Melahat Bagirova, Serhat Elcicek, Serap Yesilkir Baydar, Olga Nehir Oztel, Emrah Sefik Abamor, Sezen Canim Ates, Murat Topuzogullari, Sevil Isoglu Dincer, Zeynep Akdeste |
Journal | Asian Pacific journal of tropical medicine
(Asian Pac J Trop Med)
Vol. 10
Issue 9
Pg. 877-886
(Sep 2017)
ISSN: 2352-4146 [Electronic] India |
PMID | 29080616
(Publication Type: Journal Article)
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