Nanoparticles prepared from porcine cells support the healing of cutaneous inflammation in mice and wound re-epithelialization in human skin.

Abstract

Previous reports have demonstrated that cell-derived nanoparticles (CDNPs) composed of bovine or porcine protein complexes exerted therapeutic effects against viral infections and cancer in mice and humans. Based on these observations, we asked whether CDNPs would improve inflammatory skin disorders. To address this, we utilized two distinct mouse models of cutaneous inflammation: the autoimmune skin-blistering disease epidermolysis bullosa acquisita (EBA) as an example of an autoantibody-induced cutaneous inflammation, and Leishmania major (L. major) infection as an example of a pathogen-induced cutaneous inflammation. In both models, we observed that CDNPs increased mRNA expression of the Th2 cytokine IL-4. Clinically, CDNPs decreased inflammation due to EBA and increased L. major-specific IgG1 levels without major effects on infected skin lesions. In addition, CDNPs supported the growth of keratinocytes in human skin cultures. In vitro studies revealed that CDNPs were taken up predominantly by macrophages, leading to a shift towards the expression of anti-inflammatory cytokine genes. Altogether, our data demonstrate that treatment with porcine CDNPs may be a new therapeutic option for the control of autoimmune-mediated inflammatory skin disorders.

• Authors: Natalia Kunz, Eva Hauenschild, Sebastian Maass, Kai-Uwe Kalies, Matthias Klinger, Melanie Barra, Lars Hecht, Franz Helbig, Stefan Soellner, Charles C Caldwell, Ralf J Ludwig, Jürgen Westermann, Kathrin Kalies
• Journal: Experimental dermatology (Exp Dermatol) Vol. 26 Issue 12 Pg. 1199-1206 (12 2017) ISSN: 1600-0625 [Electronic] Denmark
• PMID: 28940860 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Chemical References

• Interleukin-4

Topics

• Adult
• Animals
• Cell Differentiation
• Cell-Derived Microparticles (transplantation)
• Epidermolysis Bullosa Acquisita (immunology, therapy)
• Female
• Humans
• Interleukin-4 (metabolism)
• Leishmania major
• Leishmaniasis, Cutaneous (immunology, therapy)
• Lymph Nodes (metabolism)
• Macrophages (metabolism)
• Mice
• Mice, Inbred C57BL
• Middle Aged
• Re-Epithelialization
• Swine
• Th2 Cells (physiology)
• Young Adult