In Vitro Dialysis of Cytokine-Rich Plasma With High and Medium Cut-Off Membranes Reduces Its Procalcific Activity.

Abstract

Recently developed high-flux (HF) dialysis membranes with extended permeability provide better clearance of middle-sized molecules such as interleukins (ILs). Whether this modulation of inflammation influences the procalcific effects of septic plasma on vascular smooth muscle cells (VSMCs) is not known. To assess the effects of high cut-off (HCO) and medium cut-off (MCO) membranes on microinflammation and in vitro vascular calcification we developed a miniature dialysis model. Plasma samples from lipopolysaccharide-spiked blood were dialyzed with HF, HCO, and MCO membranes in an in vitro miniature dialysis model. Afterwards, IL-6 concentrations were determined in dialysate and plasma. Calcifying VSMCs were incubated with dialyzed plasma samples and vascular calcification was assessed. Osteopontin (OPN) and matrix Gla protein (MGP) were measured in VSMC supernatants. IL-6 plasma concentrations were markedly lower with HCO and MCO dialysis. VSMC calcification was significantly lower after incubation with MCO- and HCO-serum compared to HF plasma. MGP and OPN levels in supernatants were significantly lower in the MCO but not in the HCO group compared to HF. In vitro dialysis of cytokine-enriched plasma samples with MCO and HCO membranes reduces IL-6 levels. The induction of vascular calcification by cytokine-enriched plasma is reduced after HCO and MCO dialysis.

• Authors: Kevin Willy, Michael Hulko, Markus Storr, Rose Speidel, Julia Gauss, Ralf Schindler, Daniel Zickler
• Journal: Artificial organs (Artif Organs) Vol. 41 Issue 9 Pg. 803-809 (Sep 2017) ISSN: 1525-1594 [Electronic] United States
• PMID: 28524237 (Publication Type: Journal Article)
• Copyright: © 2017 International Center for Artificial Organs and Transplantation and Wiley Periodicals, Inc.

Chemical References

• Calcium-Binding Proteins
• Extracellular Matrix Proteins
• Hemodialysis Solutions
• IL6 protein, human
• Interleukin-6
• Membranes, Artificial
• SPP1 protein, human
• Osteopontin

Topics

• Adolescent
• Calcium-Binding Proteins (blood, chemistry)
• Cells, Cultured
• Extracellular Matrix Proteins (blood, chemistry)
• Healthy Volunteers
• Hemodialysis Solutions (chemistry)
• Humans
• In Vitro Techniques
• Inflammation (blood, complications)
• Interleukin-6 (blood, chemistry)
• Kidney Failure, Chronic (therapy)
• Membranes, Artificial
• Myocytes, Smooth Muscle
• Osteopontin (blood, chemistry)
• Plasma (chemistry, microbiology)
• Renal Dialysis (adverse effects, instrumentation, methods)
• Vascular Calcification (blood, etiology, prevention & control)
• Matrix Gla Protein