Abstract | BACKGROUND:
Allergic contact dermatitis (ACD) is associated with increased production of cytokines. The patch test is the "gold-standard" diagnostic method, but it poses a risk of false results. OBJECTIVE: To evaluate a novel laboratory technique, the Luminex LiquiChip, which simultaneously measures blood levels of multiple cytokines, as a diagnostic tool in patients with chrome-induced ACD. METHODS: RESULTS:
Potassium dichromate-stimulated PBMCs secreted significantly higher amounts of all cytokines except TNF-α than nonstimulated PBMCs. PBMCs from patients with ACD to chromium secreted significantly higher amounts of all cytokines tested, except IL-4, compared to PBMCs from patients with ACD to nickel or fragrance. CONCLUSIONS:
Potassium dichromate stimulates the production of both Th1- and Th2-type cytokines in patients with chrome allergy. The Luminex LiquiChip is a promising in vitro method and may serve as a diagnostic tool for ACD.
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Authors | Lehavit Akerman, Tirza Klein, Moshe Israeli, Abigail Fraser, Eti Sagy, Igor Snast, Daniel Mimouni, Michael David, Akiva Trattner |
Journal | Dermatology (Basel, Switzerland)
(Dermatology)
Vol. 232
Issue 6
Pg. 748-751
( 2016)
ISSN: 1421-9832 [Electronic] Switzerland |
PMID | 28231571
(Publication Type: Journal Article)
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Copyright | © 2017 S. Karger AG, Basel. |
Chemical References |
- Coloring Agents
- Cytokines
- Potassium Dichromate
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Topics |
- Adult
- Coloring Agents
(adverse effects)
- Cytokines
(blood)
- Dermatitis, Allergic Contact
(blood, diagnosis, etiology)
- Diagnostic Techniques and Procedures
(instrumentation)
- Female
- Humans
- Male
- Middle Aged
- Patch Tests
- Potassium Dichromate
(adverse effects)
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