Abstract | PROBLEM: Increasing evidence demonstrates that inflammatory cytokines are involved in LPS-induced adverse pregnant outcomes including early embryo loss. Vitamin D3 (VitD3) has anti-inflammatory activity. We aimed to investigate the effects of vitamin D3 (VitD3) on LPS-induced early embryo loss in mice. METHOD OF STUDY: All pregnant mice except controls were intraperitoneally (ip) injected with LPS on GD7. In VitD3 alone and LPS+VitD3 groups, pregnant mice were pretreated with VitD3 by gavage daily from GD5 to GD7. RESULTS: LPS caused 62.5% pregnant mice with early embryo loss. Interestingly, the rate of abortion dropped to 14.3% when pregnant mice were pretreated with VitD3. Additional experiment showed that VitD3 significantly attenuated LPS-evoked elevation on TNF-α, IFN-γ, MIP-2, and nitrate plus nitrite in maternal serum. In addition, VitD3 alleviated LPS-induced COX-2 expression in the decidua and attenuated the elevation of PGF2α in maternal serum. Although VitD3 had no effect on IL-10 in maternal serum, it induced further elevation of serum IL-10 level in LPS-treated mice. Further analysis showed that VitD3 activated VDR signaling, simultaneously inhibited LPS-induced nuclear translocation of NF-κB p65 subunits in the decidua. CONCLUSIONS: VitD3 protects mice from LPS-induced early embryo loss at least partially through its anti-inflammatory effects.
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Authors | Yan Zhou, Yuan-Hua Chen, Lin Fu, Zhen Yu, Mi-Zhen Xia, Xiao-Guang Hu, Hua Wang, De-Xiang Xu |
Journal | American journal of reproductive immunology (New York, N.Y. : 1989)
(Am J Reprod Immunol)
Vol. 77
Issue 3
(Mar 2017)
ISSN: 1600-0897 [Electronic] Denmark |
PMID | 28045211
(Publication Type: Journal Article)
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Copyright | © 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. |
Chemical References |
- Anti-Inflammatory Agents
- Cytokines
- Inflammation Mediators
- Lipopolysaccharides
- NF-kappa B
- Cholecalciferol
- Dinoprost
- Ptgs2 protein, mouse
- Cyclooxygenase 2
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Topics |
- Administration, Oral
- Animals
- Anti-Inflammatory Agents
(immunology)
- Cholecalciferol
(immunology)
- Cyclooxygenase 2
(metabolism)
- Cytokines
(blood)
- Decidua
(metabolism)
- Dinoprost
(blood)
- Embryo Loss
(immunology, prevention & control)
- Female
- Humans
- Inflammation
(immunology)
- Inflammation Mediators
(blood)
- Lipopolysaccharides
(immunology)
- Mice
- Mice, Inbred ICR
- NF-kappa B
(metabolism)
- Pregnancy
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